GMP Recombinant Human GM-CSF (carrier-free)

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Other Names
Colony stimulating factor 2, CSF2, Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA).
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Human_GM-CSF_CF_GMP-RECOM_1_090220
GMP recombinant human GM-CSF induces dose-dependent proliferation of TF-1 erythroleukemic cells. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. The ED50 range for this effect is 0.01 – 0.04 ng/mL.
  • Human_GM-CSF_CF_GMP-RECOM_1_090220
    GMP recombinant human GM-CSF induces dose-dependent proliferation of TF-1 erythroleukemic cells. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. The ED50 range for this effect is 0.01 – 0.04 ng/mL.
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572914 25 µg 287€
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572916 100 µg 924€
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Description

GM-CSF plays a key role in signaling emergency hemopoiesis (predominantly myelopoiesis) in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. The receptor for GM-CSF is a heterodimer that comprises a major binding subunit (GMRα) and a major signaling subunit (βc). The receptor subunits are always co-expressed on the surface of leukocytes, with βc being expressed at lower levels than GMRα. Certain non-hemopoietic cell types have also been reported to express the GM-CSF receptor and to respond to GM-CSF stimulation in vitro, although the in vivo significance of these observations remains uncertain.

Product Details
Technical data sheet

Product Details

Source
Human GM-CSF, amino acids Ala18- Glu144 (Accession# NM_000758) was expressed in E. coli.
Molecular Mass
The 127 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 14.5 kD. The DTT-reduced protein migrates at approximately 14 kD and the non-reduced protein migrates with slightly greater mobility by SDS-PAGE.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.1 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method
Concentration
500 µg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.01 – 0.04 ng/mL as determined by the dose-dependent stimulation of TF-1 cell proliferation. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation.
Application

Bioassay
Cell Culture

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

BioLegend GMP recombinant protiens are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
Cytokine
Distribution

T cells, macrophages, fibroblasts, endothelial cells, mast cells.

Function
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Interaction
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts, dendritic cells, T cells.
Ligand/Receptor
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common
Bioactivity
Measured by its ability to induce proliferation of TF-1 erythroleukemic cells.
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Hercus TR, et al. 2009. Blood. 114:1289-98.
  2. Hayashida K, et al. 1990. Proc Natl Acad Sci USA. 87:9655-9.
  3. Walker F & Burgess AW. 1985. EMBO J. 4:933-9.
  4. Kitamura T, et al. 1989. J Cell Physiol. 140:323-34.
Gene ID
1437 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.16.2021

For research and further manufacturing use. Not for diagnostic or therapeutic procedures. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to reverse engineer functionally similar materials without written approval of BioLegend. By use of these products you accept the terms and conditions of any and all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research and further manufacturing use only and not intended for human or animal diagnostic or therapeutic use.

 

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