FITC anti-mouse CD90.2 (Thy1.2) Antibody

Pricing & Availability
30-H12 (See other available formats)
Regulatory Status
Other Names
Rat IgG2b, κ
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Product Citations
C57BL/6 thymocytes stained with 30-H12 FITC
  • 30-h12
    C57BL/6 thymocytes stained with 30-H12 FITC
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105305 50 µg 44€
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105306 500 µg 215€
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CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse thymus or spleen
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
  3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
  4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
  5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
  6. Hardy B, et al. 2005. Int. Immunol. 17:615.
  7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  9. Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations
  1. Yang AC, et al. 2020. Nature. 583:425. PubMed
  2. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  3. He Y, et al. 2021. Infect Immun. 89:. PubMed
  4. Wang D, et al. 2018. Immunity. 48:659. PubMed
  5. Silva C, et al. 2019. Cell Physiol Biochem. 52:503. PubMed
  6. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  7. Chimenti I, et al. 2022. Cell Death Discov. 8:149. PubMed
  8. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  9. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  10. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  11. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  12. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  13. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  14. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
  15. Westbrook A, et al. 2012. Mutagenesis. 27:77. PubMed
  16. Zhang Y, et al. 2022. Vaccines (Basel). 10:. PubMed
  17. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  18. Yang P et al. 2019. Cell Syst. 8(5):427-445 . PubMed
  19. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  20. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  21. Ni T, et al. 2016. Nat Cell Biol. 18:1221-1232. PubMed
  22. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  23. Sugii S, et al. 2010. Proc Natl Acad Sci U S A. 107:3558. PubMed
  24. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  25. Tanaka S, et al. 2021. Am J Transplant. 21:353. PubMed
  26. Kaji T, et al. 2016. Int Immunol. 28: 267 - 282. PubMed
  27. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  28. Hu J, et al. 2022. Stem Cell Res Ther. 13:349. PubMed
  29. Tanaka K, et al. 2009. Cell Stem Cell. 4:217. PubMed
  30. Barwick BG, et al. 2018. Nat Commun. 9:1900. PubMed
  31. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
AB_313176 (BioLegend Cat. No. 105305)
AB_313177 (BioLegend Cat. No. 105306)

Antigen Details

Ig superfamily, 25-35 kD

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Cell Type
Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.

Gene ID
21838 View all products for this Gene ID
View information about CD90.2 on

Related FAQs

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Other Formats

View All CD90.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD90.2 (Thy1.2) 30-H12 FC
FITC anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PE anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Purified anti-mouse CD90.2 (Thy1.2) 30-H12 FC,IHC-F,Costim,Depletion
APC anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PE/Cyanine5 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2) 30-H12 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD90.2 (Thy1.2) 30-H12 FC,IHC-F
Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PerCP anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Pacific Blue™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PE/Cyanine7 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
APC/Cyanine7 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 570™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 785™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Purified anti-mouse CD90.2 (Thy1.2) (Maxpar® Ready) 30-H12 FC,CyTOF®
Brilliant Violet 510™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PerCP/Cyanine5.5 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 605™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PE/Dazzle™ 594 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
TotalSeq™-A0075 anti-mouse CD90.2 (Thy1.2) 30-H12 PG
APC/Fire™ 750 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 711™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Ultra-LEAF™ Purified anti-mouse CD90.2 (Thy1.2) 30-H12 FC,Depletion,Costim,IHC
TotalSeq™-C0075 anti-mouse CD90.2 (Thy1.2) 30-H12 PG
TotalSeq™-B0075 anti-mouse CD90.2 (Thy1.2) 30-H12 PG
PE/Fire™ 810 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Go To Top Version: 1    Revision Date: 11.30.2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD90.2 (Thy1.2)

  • FITC anti-mouse CD90.2 (Thy1.2)

  • PE anti-mouse CD90.2 (Thy1.2)

  • Purified anti-mouse CD90.2 (Thy1.2)

  • APC anti-mouse CD90.2 (Thy1.2)

  • PE/Cyanine5 anti-mouse CD90.2 (Thy1.2)

  • Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2)

  • Alexa Fluor® 647 anti-mouse CD90.2 (Thy1.2)

  • Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2)

  • PerCP anti-mouse CD90.2 (Thy1.2)

  • Pacific Blue™ anti-mouse CD90.2 (Thy1.2)

  • PE/Cyanine7 anti-mouse CD90.2 (Thy1.2)

  • APC/Cyanine7 anti-mouse CD90.2 (Thy1.2)

  • Brilliant Violet 570™ anti-mouse CD90.2 (Thy1.2)

  • Brilliant Violet 785™ anti-mouse CD90.2 (Thy1.2)

  • Purified anti-mouse CD90.2 (Thy1.2) (Maxpar® Ready)

  • Brilliant Violet 510™ anti-mouse CD90.2 (Thy1.2)

  • PerCP/Cyanine5.5 anti-mouse CD90.2 (Thy1.2)

  • Brilliant Violet 605™ anti-mouse CD90.2 (Thy1.2)

  • PE/Dazzle™ 594 anti-mouse CD90.2 (Thy1.2)

  • Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2)

  • TotalSeq™-A0075 anti-mouse CD90.2 (Thy1.2)

  • APC/Fire™ 750 anti-mouse CD90.2 (Thy1.2)

  • Brilliant Violet 711™ anti-mouse CD90.2 (Thy1.2)

  • Ultra-LEAF™ Purified anti-mouse CD90.2 (Thy1.2)

  • TotalSeq™-C0075 anti-mouse CD90.2 (Thy1.2)

  • TotalSeq™-B0075 anti-mouse CD90.2 (Thy1.2)

  • PE/Fire™ 810 anti-mouse CD90.2 (Thy1.2)


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