FITC anti-human SSEA-4 Antibody

Pricing & Availability
Clone
MC-813-70 (See other available formats)
Other Names
Stage-Specific Embryonic Antigen-4, SSEA4
Isotype
Mouse IgG3, κ
Ave. Rating
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Product Citations
publications
MC-813-70_FITC_090208.jpg
NCCIT (human teratocarcinoma cell line) stained with MC-813-70 FITC
  • MC-813-70_FITC_090208.jpg
    NCCIT (human teratocarcinoma cell line) stained with MC-813-70 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
330409 25 tests 96€
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330410 100 tests 196€
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Description

The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Baboon, Cynomolgus, Mouse, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Kannagi R, et al. 1983. The EMBO J. 2:2355
  2. Guven S, et al. 2015. Stem Cell Transl Med. 4:261. PubMed
Product Citations
  1. Guven S, et al. 2015. Stem Cells Trans Med. 4:261. PubMed
  2. Ren Y, et al. 2018. Sci Rep. 8:7780. PubMed
Publication Library
RRID
AB_1089205 (BioLegend Cat. No. 330409)
AB_1089204 (BioLegend Cat. No. 330410)

Antigen Details

Structure
Carbohydrate epitope on glycosphingolipids
Distribution
Human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), preimplantation embryos, erythrocytes and erythromyeloid leukemia cell line K562; mouse oocytes, early cleaved embryos
Function
Regulation of differentiation
Cell Type
Erythrocytes, Mesenchymal Stem Cells, Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Kannagi R, et al. 1983. The EMBO J. 2:2355
2. Henderson JK, et al. 2002. Stem Cells. 20:329
3. Brimble SN, et al. 2007. Stem Cells. 25:54
4. Battula VL, et al. 2007. Differentiation. 75:279

Gene ID
330401 View all products for this Gene ID
UniProt
View information about SSEA-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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