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This antibody is effective in immunohistochemistry (IHC).
The BRST-1, clone CU-18, monoclonal antibody detects BCA-225, a 225 kD glycoprotein secreted by the T47D breast carcinoma cell line. Strong intracytoplasmic staining is seen in primary and metastatic breast carcinoma cell lines, primary and metastatic breast carcinoma tissue and cervical carcinomas.
Cat. No. 915001 (Formerly: SIG-3610-1000) [1 mL]
- Product Information
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.
The monoclonal antibody CU-18 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of BRST-1. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
- This antibody was generated against a protein secreted by the T47D (clone 11) human breast carcinoma cell line.
- Phosphate-buffered solution with BSA + 0.1% NaN3.
- The antibody was purified by affinity chromatography.
- Storage & Handling
- Store between 2°C and 8°C.
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Positive tissue (human): Breast carcinoma
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20 minutes at room temperature
(PubMed link indicates BioLegend citation)
- Imam SA, et al. Comparison of two microwave based antigen retrieval solutions in unmasking epitopes in formalin-fixed tissue for immunostaining. Anticancer Res 15:1153-8, 1995.
- Ansai S, et al. An immunohistochemical study of BCA-225 in various skin cancers. J Dermatol 21(1): 20-4, 1994.
- Ma CK, et al. Comparative immunohistochemical study of primary and metastatic carcinomas of the liver. Am J Clin Pathol 99(5):551-7, 1993.
AB_2565159 (BioLegend Cat. No. 915001)
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.
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