Biotin anti-human FGF-basic Antibody

Pricing & Availability
Clone
JKFb-2 (See other available formats)
Regulatory Status
RUO
Other Names
Fibroblast Growth Factor-basic, bFGF, FGF-2, Heparin-binding growth factor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
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508702 500 µg 334€
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Description

Fibroblast growth factor-basic (FGF-b, FGF-2) is a heparin-binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo. FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. The JKFb-2 antibody reacts with human fibroblast growth factor - basic (FGF-basic).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human bFGF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human FGF-basic protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate.It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated JKFb-2 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified BHFB15 antibody (Cat. No. 514801/514802) as the capture antibody.

RRID
AB_315527 (BioLegend Cat. No. 508702)

Antigen Details

Structure
FGF; 17.2 kD (Mammalian)
Bioactivity
Modulator of cell proliferation, motility, differentiation, angiogenesis; affinity for heparin; associated with ECM
Cell Sources
Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, epithelial cells
Cell Targets
Fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, astrocytes, oligodendrocytes, smooth muscle
Receptors
FGFR-1 (flg); FGFR-2 (bek, K-sam); FGFR-4 (flg-2); low affinity coreceptor heparin sulfate and heparin sulfate proteoglycans required for full activity
Biology Area
Angiogenesis, Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Klagsbrun M. 1992. Semin. Cancer Biol. 3:81.
3. Goldfarb M. 1990. Cell Growth Differ. 1:439.
4. Sensenbrenner M. 1993. Prog. Neurobiol. 41:683.

Regulation
Released after tissue injuries and during inflammatory processes
Gene ID
2247 View all products for this Gene ID
UniProt
View information about FGF-basic on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All FGF-basic Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human FGF-basic JKFb-2 ELISA Detection,ELISPOT Detection
Go To Top Version: 2    Revision Date: 11.20.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human FGF-basic

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