- Clone
- 1B1-B2 (See other available formats)
- Other Names
- Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
- Isotype
- Mouse IgG3
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/ml anti-Histone H3 (clone 1B1-B2) Alexa Fluor® 594 (red), and 5 µg/ml of anti-mouse CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 488 (green) at 4°C overnight. Nuclei were counterstained with DAPI (purple). The image was captured by 10X objective. -
TE-71 cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml anti-Histone H3 (clone 1B1-B2) Alexa Fluor® 594 (red) at 4°C overnight. The following day the cells were stained with Flash Phalloidin™ NIR 647 (green) for 20 minutes in room temperature. Nuclei were counterstained with DAPI (purple). The image was captured with a 60X objective.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 819405 | 25 µg | 100€ | ||||
| 819406 | 100 µg | 252€ | ||||
Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.
Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.
Product Details
- Reactivity
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide; may contain stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
IHC-F, IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1.25 - 5 μg/ml is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.25 - 5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Reactivity to Drosophila was only verified with the purified format.
- RRID
-
AB_2715800 (BioLegend Cat. No. 819405)
AB_2715803 (BioLegend Cat. No. 819406)
Antigen Details
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
- Gene ID
- 8350 View all products for this Gene ID
- UniProt
- View information about Histone H3 (C-terminus) on UniProt.org
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