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A single cell suspension from pooled mouse spleen and lymph nodes was processed with MojoSort™ Buffer as indicated in the protocol for isolation of CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit with the MojoSort™ Magnet. The purity was checked by staining with PE anti-mouse CD4 (clone RM4-4) and APC anti-mouse CD3ε (clone 145-2C11). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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480017 | 250 mL | 53€ |
MojoSort™ Buffer is formulated with high quality reagents to help reduce non-specific binding when using the MojoSort™ Magnetic Separation System.
Product DetailsProduct Details
- Storage & Handling
- The buffer should be stored between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. The 5X concentrate is filtered through a 0.2 µm membrane. Use the buffer under aseptic conditions as needed.
- Application Notes
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MojoSort™ Buffer is recommended for cell separation using MojoSort™ nanobeads and kits. To use the buffer, follow the protocol provided with the MojoSort™ product you are using.
Composition:- 5X Phosphate buffered saline (PBS), pH 7.2
- 2.5 % (w/v) Bovine Serum Albumin (BSA)
- 10 mM Ethylene Diamine Tetra-acetic acid (EDTA)
- Product Citations
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Antigen Details
- Gene ID
- NA
Related Pages & Pathways
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Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
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No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
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Please contact our technical service team for further assistance.
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