As the COVID-19 pandemic continues to affect us all, BioLegend is here to help the scientific community with our products, resources, and support. We understand that science is critical for finding solutions to the current situation. BioLegend is committed to supporting researchers in their efforts to characterize all aspects of the SARS-CoV-2 virus and COVID-19 disease, including the development of novel therapeutics. Our reagents are being employed in several research areas, including:

 

  • Characterization of the immune response in COVID-19 patients and disease models
  • The development and validation of viral detection kits
  • Therapeutics response monitoring
  • Monitoring vaccine candidates
  • Discovery of virus-associated host biomarkers

 

To make it easier to find what you need, we’ve compiled our relevant products based on workflow. BioLegend is here to partner with you and your teams, so if you can’t find a specific product or require a custom reagent, please reach out. Together, we can find a solution.

 

Please note: BioLegend is deemed an essential business and is operational.  If you’re looking for more information regarding our operations during this time, read a message from our CEO and find answers to our most frequently asked questions.

 

BioLegend wants to help in your fight against COVID-19

 

BioLegend is ready to collaborate with your organization to help you accelerate the development of diagnostic tests, therapeutics, and vaccines for SARS-CoV-2, and be a partner in your research. Please tell us how we can support your work.

 

SARS-CoV-2 Workflow

Click on a section in the image to scroll down to your area of interest.

Covid Workflowviral recognitioninflammation responsepathways biomarker

 


Viral Recognition

 

In order for viruses to infect a host cell, they first must recognize a protein or receptor on the host cell surface. This process is called viral recognition. In the case of SARS-CoV-2, which causes the disease COVID-19, the virus recognizes the enzyme ACE2. Specifically, the S1 spike protein of SARS-CoV-2 binds the enzymatic domain of ACE2. Once S1 and ACE2 interact, the virus enters the host cell, mediated by the TMPRSS2 protease. BioLegend provides specific SARS-CoV-2 and host recombinant proteins and antibodies to help investigate this step of the disease.

 

 

Anti-human ACE2 Antibody

Anti-SARS-CoV-2 S Protein S1 Antibody

Recombinant Human ACE2

Recombinant Mouse ACE2

Recombinant SARS-CoV-2 S Protein RBD

Recombinant SARS-CoV-2 S Protein S1

 

 


 

Characterize Virus-Induced Inflammation

 

Characterize Virus-Induced InflammationSARS-CoV-2 causes the disease COVID-19, which can result in inflammation. We provide a number of tools for researchers to detect and characterize this inflammation. Our ELISA Sets and Kits are trusted to accurately quantitate cytokine and chemokine levels, while our LEGENDplex™ kits offer a flow cytometry-based method to analyze up to 13 different targets simultaneously.

Monitor Cellular Response

 

Monitor Cellular ResponseThe cellular response to SARS-CoV-2 is key to the induced cytokines, inflammation, and recovery. Players include CD8+ T cells that possess potent cytotoxic abilities, and CD4+ Th1 cells that are known for their role in supporting anti-viral immunity and sparking an adaptive immune response. We provide reliable reagents for phenotyping and isolating cell populations of interest, including T cells, B cells, NK cells, monocytes, and more.

Flow Cytometry: our comprehensive portfolio will help characterize any cell type, including those relevant to study anti-viral and SARS-CoV-2 immune responses.

 

TotalSeq™ Reagents for Proteogenomics can be used to characterize single cells in a high throughput manner. These antibody-oligo conjugates contain unique nucleotide barcodes that integrate seamlessly into existing workflows, enabling you to simultaneously analyze protein and RNA expression on a single cell level. TotalSeq™ is available as individual or pre-titrated, lyophilized antibody cocktails, or hashtags for multiplexing samples. Specifically, they can be used to enable:

 

  • Multiomic analysis (Protein + RNA) of immune cells and lung alveolar lavage in COVID-19 patients (TotalSeq™-A or B in combination with 10x Genomics Single Cell Expression Solution (3’ assay)).
  • TCR/BCR repertoire profiling in COVID-19 patients for immunotherapy and vaccine development (TotalSeq™-C with 10x Genomics Immune Profiling Solution (5’ assay)).

 

Flex-T™ MHC Tetramers allow peptides of interest to be swapped into the binding site. This can help track the T cell response when identifying potential vaccine candidates. View Flex-T™ reagents.

Flex-T Diagram

 

View all specific SARS-CoV-2 relevant HLA monomers

 

Cell Differentiation: For mouse cells, we provide a full protocol on how to differentiate Th1 cells with our reagents, including T cell-stimulating antibodies for CD3 and CD28, recombinant proteins (IL-2, IL-12p70) and neutralizing antibodies for IL-4 to prevent Th2 differentiation.

 

View the Th1 activation bundle.

Naïve BALB/c mice CD4+ T cells data image
Naïve BALB/c mice CD4+ T cells were polarized with anti-mouse CD3, anti-mouse CD28, IL-12, IL-2, and anti-mouse IL-4 for 5 days and fresh media was added on day 3. On day 5, the cells were re-stimulated with PMA and ionomycin in the presence of monensin for 5 hours. Cells were surface stained with CD4 PerCP-Cy5.5, fixed, permeabilized and stained with IFN-γ FITC, IL-4 APC, and IL-17 PE.

 

 

MojoSort™ Kits allow you to use magnetic nanoparticles to quickly isolate cells of interest with high purity and yield. Based on our testing, cells isolated with MojoSort™ reagents retain functionality for downstream applications.

 

T cells:

CD3 Kits: Human and Mouse

CD4 Kits: Human and Mouse

CD8 Kits: Human and Mouse

B cells: Human and Mouse

NK cells: Human and Mouse

Monocytes: Human

MojoSort product lineup

View more MojoSort™ Kits and protocols.

 

 

Recombinant Cytokines and Growth Factors are also critical for cell culture and differentiation. Explore our recombinant proteins useful for cell culture. Additionally, several critical proteins have already been identified. For example, ACE2 has been demonstrated to be the host portal entry. DPP4/CD26 and Cathepsins have been used as well to study the viral mechanism for cell invasion1, 2, 3.

 

For those looking to detect various classes of human immunoglobulins, we also offer a selection of anti-human secondary reagents, which could be used to detect virus specific antibodies.


Analyze Anti-Viral Pathways

 

Understanding the anti-viral signaling pathways that respond to infection by SARS-CoV-2 is critical to the development of novel therapeutics. We offer many antibodies to detect important mediators involved in immune pathways. These include recognition of viral pathogens by Toll-like receptors, downstream signaling through STAT and NF-κB signaling, and ultimately, the production of interferons through IRF-mediated transcription. Our anti-viral pathway reagents have been validated across a variety of applications, including western blotting, flow cytometry, functional assays, ChIP, and microscopy.

Biomarker Discovery and Monitoring

 

To better understand the changes that SARS-CoV-2 can induce in cells (both through infection and the resultant host immune response), it is important to be able to screen cells for both protein and gene expression levels.

 

To support this need, our TotalSeq™ reagents provide an unprecedented resolution. The capacity to correlate protein expression with RNA and TCR/BCR profiling in single cells simultaneously can greatly accelerate the discovery of therapeutically relevant biomarkers. View our TotalSeq™ products.

 

 

In addition, LEGENDScreen™ kits offer a rapid screening method for cellular samples. Kits include several 96-well plates with each well containing a different lyophilized PE-conjugated antibody useful for staining cells. Our human kit contains antibodies for 361 cell surface markers and 10 isotype controls). Our mouse kit contains 255 antibodies for cell surface markers and 11 isotype controls. View our LEGENDScreen™ products.

 

 


BioLegend wants to help in your fight against COVID-19
 

BioLegend is ready to collaborate with your organization to help you accelerate the development of diagnostic tests, therapeutics, and vaccines for SARS-CoV-2, and be a partner in your research. Please tell us how we can support your work.

 


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