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SARS-CoV-2 Reactive T cell Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Lymphocyte function-associated antigen 1, LFA-1, antigen specific, T cells, CD11a/CD18
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A_345903_SARSCoV2_Reactive_T_cell_kit
Kit content image for the SARS-CoV-2 Reactive T cell Kit
  • A_345903_SARSCoV2_Reactive_T_cell_kit
    Kit content image for the SARS-CoV-2 Reactive T cell Kit
  • B_SARS-CoV-2-Reactive_T-cell-Kit_042922
    CD8+ human peripheral blood lymphocytes were stimulated lyophilized negative control (left) or SARS-CoV-2 peptide pool (right) for 20 minutes, then stained with anti-human LFA-1 PE followed by surface cocktail. Data was analyzed using gating strategy depicted in Figure below.
  • C_SARS-CoV-2-Reactive_T-cell-Kit__042722
    CD4+ human peripheral blood lymphocytes were stimulated with lyophilized negative control (left) or SARS-CoV-2 peptide pool (right) for 4 hours, then stained with anti-human LFA-1 PE followed by surface cocktail. Data was analyzed using gating strategy depicted in Figure below.
  • D_SARS-CoV-2-Reactive_T-cell-Kit__042722
    Gating strategy using scatter parameters and surface markers in the staining cocktail to identify CD4+ and CD8+ cells.
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345903 10 tests $464
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Description

The SARS-CoV-2 reactive T cell kit contains SARS-CoV-2 peptide pool (tube S), negative control (tube N) and positive control (tube P) in a convenient packaging for stimulating human blood/ PBMCs along with anti-human LFA-1 PE and a surface stain cocktail consisting of anti-human CD45 PerCP, CD8 Pacific Blue™, CD4 FITC and CD14, CD41, CD15, CD16, CD19 APC/Cyanine7. The kit can be used for assessing both SARS-CoV-2 specific responses and vaccine responses. Cells or beads stained with individual fluorochrome-conjugated antibodies should be used for setting up compensation before running the samples. For data analysis, we recommend following gating strategy – Time –> Singlets –> Lymphocytes –> APC/Cyanine7 (Dump) negativeCD45+ –> CD4+CD8– and CD8+CD4–.

The SARS-CoV-2 reactive T cell kit provides a convenient, easy to use, and powerful tool for immunology and vaccine research.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Formulation
Each kit will contain the following components:

Kit Components:

1 vial negative control
1 vial positive control
1 vial SARS-CoV-2 Spike (Glycoprotein) peptide pool
1 vial surface stain cocktail (5 tests)
3 tubes of LFA-1 PE (1 test each)
1 bottle 50ml RBC Lysis/Fixation solution
1 bottle 0.5ml EDTA 0.08M
1 bottle 500ml Cell Staining Buffer
Preparation
Kit consists of lyophilized components.
Storage & Handling
Store the kit at 2°C - 8°C upon receipt. Do not open the sealed pouch until ready to use. One pouch contains sufficient reagents to test one donor sample.
Application

FC - Quality tested
Recommended Usage

Refer to manual for detailed usage instructions.

Application References

(PubMed link indicates BioLegend citation)
  1. Dimitrov S, et al. 2018. Pro Natl Acad Sci  U S A. 115:E5536-E5545.
  2. Gouttefageas C, et al. 2019. Cancer Immunol Immunother. 68:1855-1863.
  3. Schollhorn A, et al. 2021. Front Immunol. 12:626308.
  4. Schollhorn A, et al. 2023. Front Immunol. 13:1107366.

Antigen Details

Interaction
CD4+ T cells, CD8+ T cells, SARS-CoV-2, antigen specificity
Ligand/Receptor
β2-integrin
Antigen References
  1. Kundu R, et al. 2022. Nature Comm. 13:80.
  2. Lou XH, et al. 2021. Scand J Immunol. 93:e12989.
  3. Canete PF, et al. 2020. Cell. 183:13.
  4. Sette A, et al. 2021. Cell. 184:861.
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/27/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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