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Ultra-LEAF™ Purified anti-mouse CXCL7 (NAP-2) Antibody

Pricing & Availability
Clone
A15073B (See other available formats)
Regulatory Status
RUO
Other Names
Neutrophil activating protein 2 (NAP2)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A15073B_ULEAF_CXCL7_Antibody_012722
Recombinant mouse CXCL7 (NAP-2) (Cat. No. 586704) (black circles) induces the chemotaxis of mouse Baf3-mCXCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-mouse CXCL7 (NAP-2) (clone A15073B) (purple squares) neutralizes the chemotaxis of recombinant mouse CXCL7 (NAP-2) (Cat. No. 586704) at 3 ng/mL on mouse Baf3-mCXCR2 cells in a dose-dependent manner whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 0.3 - 2 µg/mL
  • A15073B_ULEAF_CXCL7_Antibody_012722
    Recombinant mouse CXCL7 (NAP-2) (Cat. No. 586704) (black circles) induces the chemotaxis of mouse Baf3-mCXCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-mouse CXCL7 (NAP-2) (clone A15073B) (purple squares) neutralizes the chemotaxis of recombinant mouse CXCL7 (NAP-2) (Cat. No. 586704) at 3 ng/mL on mouse Baf3-mCXCR2 cells in a dose-dependent manner whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 0.3 - 2 µg/mL
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945703 100 µg 287€
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945704 1 mg 964€
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Description

CXCL7 is an ERL+ chemokine that is secreted by activated platelets, neutrophils, lymphocytes, and macrophages as a precursor. Pro-platelet basic protein or leukocyte derived grow factor (PPBP/LDGF) (a protein of ~14 kD) is modified by post-transcriptional cleavage to give rise to connective tissue activating peptide (CTAP-III), CXCL7, β-thromboglobulin (β-TG), and two variants of thrombocidin (TC-1 and TC-2). CTAP-III is activated by enzymatic proteolysis performed by cell surface-bound cathepsin G (CathG), a chymotryptic serine protease found in primary neutrophil granules. In this way, neutrophils are the mayor cells that convert CTAP-III into CXCL7. In addition, monocytes and mast cells can proteolytically modify CTAP-III to produce CXCL7. In fact, CathG has been detected in mast cells in addition to chymase, another chymotryptic enzyme. Further C-terminal truncations, that eliminate the last four and seven amino acids in CXCL7, generate two additional natural isoforms. These variants are more potent neutrophils activators. 
 

High levels of CTAP-III have been detected in plasma of patients with lung cancer. Because of this, it has been suggested as a biomarker for early lung cancer detection. An increase in positive immunostained cells has been detected in the bronchial submucosa of patients with stable severe chronic obstructive pulmonary disease. In addition, studies with premalignant breast cancer cells transfected with CXCL7 showed that these cells became as invasive as malignant breast cancer cells. CXCL7 also induces stimulation of the lymphangiogenic factors VEGF-C and VEGF-D in human breast cancer cells, suggesting an important role for CXCL7 in tumor invasion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CXCL7
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CXCL7 (NAP-2) (Cat. No. 586704) (3 ng/mL) on mouse Baf3-mCXCR2 transfectant chemoattractant cells. ND50 range: 0.3 - 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910505 (BioLegend Cat. No. 945703)
AB_2910505 (BioLegend Cat. No. 945704)

Antigen Details

Structure
Chemokine
Distribution

Platelets, neutrophils, lymphocytes, macrophages, endothelial cells

Function
CXCL7 stimulates chemotaxis and degranulation in neutrophils, induces transendothelial migration and neutrophil adhesion to activated endothelial cells, and has angiogenic activity. LPS induces the precursor in PBMCs
Interaction
Neutrophils, keratinocytes
Ligand/Receptor
CXCR2
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Iida N, et al. 1996. FASEB J. 10:1336-45.
  2. Schenk BI, et al. 2002. J Immunol. 169:2602-10.
  3. El-Gedaily A, et al. 2004. J Leukoc Biol. 75:495-503.
  4. Schiemann F, et al. 2006. Blood. 107:2234-42.
  5. Tang Z, et al. 2008. Am J Surg. 196:690-6.
  6. Yee J, et al. 2009. J Clin Oncol. 27:2787-92.
  7. Yu M, et al. 2010. J Oncol. 2010:939407.
  8. Lee G, et al. 2011. Am J Transl Res. 3:226-33.
  9. Nguyen LT and Vogel HJ. 2012. Front Immunol. 3:384.

 

Gene ID
57349 View all products for this Gene ID
UniProt
View information about CXCL7 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01.19.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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