- QA20A30 (See other available formats)
- Regulatory Status
- Other Names
- B cell growth factor 1 (BCGF-1), B-cell stimulatory factor 1 (BSF-1), interleukin-4, lymphocyte stimulatory factor 1.
- Human IgG1, κ
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- Product Citations
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IL-4 is the primary cytokine implicated in the development of Th2-mediated responses, which is associated with allergy and asthma. IL-4 binds to two receptor complexes, the Type I receptor comprises IL-4Rα and the common gamma-chain (γc), which is also shared by the cytokines IL-2, -7, -9, -15 and -21 and is present in hematopoietic cells. IL-4 can use the type II complex, comprising IL-4Rα and IL-13Rα1, which is present in non-hematopoietic cells. This second receptor complex is a functional receptor for IL-13, which shares approximately 25% homology with IL-4. The type I receptor complex can be formed only by IL-4 and is active in Th2 development. In contrast, the type II receptor complex formed by either IL-4 or IL-13 is more active during airway hypersensitivity and mucus secretion and is not found in T cells. IL-4, through its activation of STAT6, upregulates GATA3 expression and also suppresses TH1 and TH17 cell responses, partly through the upregulation of growth factor independent 1(GFI1), a transcriptional repressor of IFN-γ and IL-17 production. IL-4 induces macrophage activation and TSLP production. IL-4 recruits and activates IgE-producing B cells (IgE class switching) and enhances IgE-mediated responses by up-regulating IgE receptors on B lymphocytes, mast cells, and basophils. In addition, IL-4 also induces VCAM-1 on vascular endothelium and thus directs the migration of T lymphocytes, monocytes, basophils, and eosinophils to the inflammation site.Product Details
- Antibody Type
- Host Species
- Recombinant human IL-4
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant human IL-4 (Cat. No. 766206) at 7 ng/mL on TF-1 cells. ND50 range: 0.02 - 0.1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
AB_2892511 (BioLegend Cat. No. 945303)
AB_2892511 (BioLegend Cat. No. 945304)
IL-4 is produced by Th2 cells, naive CD4+ T cells, NKT cells, and basophils.
- Major role in differentiation of TH2 cells and induction of Th2 associated cytokines, suppresses TH1 and TH17 cell responses, induces macrophage activation and TSLP production, enhances IgE-mediated in B lymphocytes, mast cells, and basophils.
- T cells, B cells, macrophages, epithelial cells, smooth muscle cells, bronchial fibroblasts
- IL-4 signals through Type I (IL-4Rα, γc) and Type II receptors (IL-4Rα, IL-13Rα1) complexes.
- Antigen References
- Swain SL, et al. 1990. J. Immunol. 145:3796-806.
- Hsieh CS, et al. 1992. Proc. Natl. Acad. Sci. USA 89:6065-9.
- Allison-Lynn A, et al. 2006. J. Immunol. 176:7456-61.
- Kato A, et al. 2007. J. Immunol. 179:1080-7.
- LaPorte SL, et al. 2008. Cell 132:259-72.
- Martinez FO, et al. 2009. Annu. Rev. Immunol. 27:451-83.
- Luzina IG, et al. 2012. J. Leuko. Biol. 92:753-64.
- Gene ID
- 3565 View all products for this Gene ID
- View information about IL-4 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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