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- Clone
- A15153G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human TIGIT.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).
Additional reported applications (for the relevant formats) include: Blocking1. - Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) -
- Stamm H, et al. 2018. Oncogene. Pubmed
Antigen Details
- Structure
- 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
- Distribution
-
Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.
- Function
- Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
- Ligand/Receptor
- CD155 (PVR), CD112 (PVRL2, NECTIN-2).
- Cell Type
- NK cells, T cells, Tfh, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923. - Gene ID
- 201633 View all products for this Gene ID
- UniProt
- View information about TIGIT on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All TIGIT (VSTM3) Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
Human PBMCs were labeled with CFSE. Cells were cultured in ... -
APC/Fire™ 750 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
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APC anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
-
PE anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 APC ... 
-
Brilliant Violet 421™ anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
-
Brilliant Violet 605™ anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
-
PE/Dazzle™ 594 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
-
PerCP/Cyanine5.5 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 APC ... -
PE/Cyanine7 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with FITC CD3... -
Ultra-LEAF™ Purified anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
Human PBMCs were labeled with CFSE. Cells were cultured in ... -
Biotin anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with CD3 FITC... 
-
Alexa Fluor® 647 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 PE ... 
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TotalSeq™-A0089 anti-human TIGIT (VSTM3)
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TotalSeq™-B0089 anti-human TIGIT (VSTM3)
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TotalSeq™-C0089 anti-human TIGIT (VSTM3)
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KIRAVIA Blue 520™ anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 APC... -
APC/Cyanine7 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 PE ... -
Brilliant Violet 510™ anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody
Human peripheral blood lymphocytes were stained with CD3 PE ... -
TotalSeq™-D0089 anti-human TIGIT (VSTM3)
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Brilliant Violet 711™ anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with CD3 Ale... -
PE/Fire™ 640 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with anti-hum... -
PE/Fire™ 810 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocyte were stained with anti-hum... -
PE/Cyanine5 anti-human TIGIT (VSTM3)
Human peripheral blood leukocytes were stained with anti-hum... -
PerCP/Fire™ 806 anti-human TIGIT (VSTM3)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human TIGIT (VSTM3)
Human lysed whole blood cells were stained with anti-human C... -
Spark Red™ 718 anti-human TIGIT (VSTM3) (Flexi-Fluor™)
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