- Regulatory Status
- Other Names
- Monocyte chemotactic protein 3 (MCP-3)
- Ave. Rating
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- Product Citations
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Mouse CCL7 was initially identified by differential screening of a cDNA library of serum stimulated NIH 3T3 cells. CCL7 has similarity with CCL2/MCP-1 (53%) and CCL8/MCP-2 (48%) at the amino acid level. Chemokines play a key role in inflammation and in the initial phase, CXC chemokines chemoattract polymorpho-nuclear leuckocytes (PMNs). The next step in the inflammatory processes is directed in part by CC chemokines including the monocyte chemoattractant proteins (CCL2, CCL7, CCL8 and CCL13) that chemoattracts additional leucocytes subsets. It has been reported that MMPs regulate chemokine bioactivity, increasing or decreasing their chemoattractant property; MMP-8 and MMP-9 modulate CXC chemokines, and MMP-2 truncates the N-terminal of CCL7 eliminating the first four amino acids producing CCL7(5-76 residues), leading to a loss of activity. The truncated CCL7 binds to its receptor, but it does not induce signaling transduction. MCPs are truncated by different MMPs to create potent antagonists and induce decrease in inflammation. For example, MMP-13 cleaves CCL2 and CCL7 and generates potent receptor antagonists. In an experimental model of myocarditis induced in MMP-2 knockout mice, an increase of CCL7 and high inflammatory response, with pronounced myocardial damage mediated by inflammatory cells, resulted from reduced degradation of CCL7 due to the absence of MMP-2.Product Details
- Mouse CCL7, amino acids Gln24-Pro97 (Accession# NM_013654.3) was expressed in E. coli.
- Molecular Mass
- The 74 amino acid recombinant protein has a predicted molecular mass of approximately 10.3 kD. The DTT-reduced protein migrates at approximately 13 kD and non-reduced protein migrates at approximately 14.5 kD by SDS-PAGE. The N-terminal amino acid is Gln.
- >98%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Mouse CCL7 chemoattracts human monocytic TH-1 cells in a dose-dependent manner. The ED50 for this effect is 20 – 80 ng/mL.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
Fibroblasts, mast cells, astrocytes, endothelial cells, monocytes, and macrophages
- CCL7 chemoattracts monocytes, T cells, NK cells, eosinophils, and basophils. CCL7 protein is secreted by mononuclear leukocytes after stimulation with IL-1β, IFN-α, IFN-β, measles virus, poly I-C, or Con A. In fibroblasts, CCL7 mRNA and protein are synergistically induced after treatment with IL-1β and IFN-γ. CCL7 is stimulated in murine fibroblasts and astrocytes by TGF-β and TNF-α respectively. Anti-inflammatory cytokines (IL-4, IL-10, or IL-13) inhibit the expression of CCL7 in monocytes.
- Monocytes, T cells, NK cells, dendritic cells, mast cells, eosinophils, and basophils
- CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195)
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Antigen References
1. Heinrich JN, et al. 1993. Mol. Cell Biol. 13:2020.
2. Menten P, et al. 1999. Eur. J. Immunol. 29:678.
3. McQuibban GA, et al. 2000. Science 289:1202.
4. Menten P, et al. 2001. Eur. Cyto. Net. 12:554.
5. Dean RA, et al. 2008. Blood 112:3455.
6. Renner NA, et al. 2011. J. Neurovirol. 17:146.
7. Feuser K, et al. 2012. Cytokine 58:178.
8. Westermann D, et al. 2011. Circulation 124:2082.
- Gene ID
- 20306 View all products for this Gene ID
- View information about CCL7 on UniProt.org
- Why choose BioLegend recombinant proteins?
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins.
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)