Recombinant Human SUMO3-GFP Protease Substrate (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Small Ubiquitin Like Modifier 3, Small Ubiquitin-Related Modifier 3, SMT3H1, SUMO-3, SMT3A
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Human_SUMO3-GFP_Protease_Substrate_CF_RECOM_1_061621.png
The ability of recombinant human SUMO3-GFP protease substrate to be cleaved by different proteases (SENP2, thrombin, ENTK, 3C, factor Xa, and TEV) is visible by an SDS-PAGE gel.
  • Human_SUMO3-GFP_Protease_Substrate_CF_RECOM_1_061621.png
    The ability of recombinant human SUMO3-GFP protease substrate to be cleaved by different proteases (SENP2, thrombin, ENTK, 3C, factor Xa, and TEV) is visible by an SDS-PAGE gel.
  • Human_SUMO3-GFP_Protease_Substrate_CF_RECOM_2_061621.png
    Stability Testing for Recombinant Human SUMO3-GFP Protease Substrate. Human SUMO3-GFP was aliquoted in 20 mM Tris pH 8.0, 100 mM NaCl. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4°C (Control). The samples were tested for their ability to be cleaved by different proteases (3C and thrombin). Cleavage of human SUMO3-GFP is visible by an SDS-PAGE gel.
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793304 25 µg 80,00€
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793306 100 µg 144,00€
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Description

The protein, SUMO3, is a member of the small ubiquitin related modifier (SUMO) family. At least three SUMO family members, SUMO1, SUMO2, and SUMO3, are found in mammals. SUMO3 shares a 42% sequence identity with SUMO1 and a 96% identity with SUMO2. After translation, the protein is modified and covalently conjugates to other proteins. This post-translation modification is referred to as sumolyation. SUMO proteins are linked to other proteins via an isopeptide bond formed between their carboxyl-terminal glycine residues and the lysine(s) of the substrate. Most of the substrate proteins that SUMO modifies are located in the nucleus. The mechanism of conjugation of SUMO with substrates parallels to the mechanism of ubiquitination. Sumolyation is integrated in many cellular processes such as DNA replication and repair, nuclear transport, signal transduction, and regulation of transcription. SUMO3 is successfully cleaved by different proteases. A family of sentrin/SUMO-specific proteases (SENPs) regulates the reversible process of sumolyation. SENP2 is a protease that de-modifies SUMO3 and other SUMO family protein conjugates. SUMO3-GFP is successfully cleaved by the following enzymes: SENP2, thrombin, enterokinase cleavage enzyme (EKT), active HRV14 3C, TEV, and factor Xa. SUMO fusion technology has been use to enhance protein expression, solubility, and purification in prokaryotes. E.coli lacks the SUMO protease, therefore the purification of SUMO-tag proteins is achievable. A different fusion system called split SUMO has been developed for the expression of SUMO-tagged proteins in eukaryotic systems.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human SUMO3, amino acid (Asn14-Gly92) (Accession # P55854.2) was expressed in pBL-2 cells. The N terminal has a Met and 6x His tag, followed by different protease cleavage [SENP2, thrombin, enterokinase, 3C protease, factor Xa, and tobacco Etch virus (TEV)] and C-terminal GFP (AAB02574.1).
Molecular Mass
The 356 amino acid recombinant protein has a predicted molecular mass of approximately 40.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 50 kD by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.2 µm filtered protein solution is in 20 mM Tris pH 8.0, 100 mM NaCl.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Human SUMO3-GFP protease substrate is cleaved successfully by SENP2, thrombin, enterokinase, 3C protease, factor Xa, and tobacco Etch virus (TEV).
Application

Bioassay

Application Notes

Human SUMO3-GFP Protocol:

Human SUMO3-GFP is digested by different proteases.

  1. Incubate mixtures of 5 µg of SUMO3-GFP with protease (eigther SENP2, thrombin, enterokinase (ENTK), 3C protease, factor Xa, TEV) for 16 hours at 25°C.
  2. Run SDS-PAGE gel with mixtures.

Assay Buffer for Each Protease Mixture:

a) Human SENP2 and 3C protease buffer: 20 mM Tris, pH8, 100 mM NaCl

b) Thrombin and factor Xa buffer: 20 mM Tris, pH 8, 100 mM NaCl, 2 mM CaCl2

c) Enterokinese buffer: 20 mM Tris, pH 8, 100 mM NaCl

d) TEV buffer: 20 mM Tris, pH 8, 100 mM NaCl, 1 mM DTT, 0.5 mM EDTA​


BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Additional Product Notes


 

Antigen Details

Structure
Monomer
Distribution

SUMO is ubiquitously expressed.

Function
Functions in post-translational modification system
Ligand/Receptor
Substrate for: SEMP2, thrombin, enterokinase, 3C protease, factor Xa and TEV
Bioactivity
SUMO3-GFP protease substrate is cleaved successfully by different proteases including: SENP2, thrombin, enterokinase, prescission 3C, factor Xa and TEV.
Cell Type
B cells, Epithelial cells, Neurons, T cells
Biology Area
Cardiovascular Biology, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Neuroscience
Molecular Family
Proteases
Antigen References
  1. Saitoh H, et al. 1997. Trends Biochem Sci. 22:374.
  2. Yeh ETH, et al. 2000. Gene. 248:1.
  3. Zhang H, et al. 2002. Mol Cell Biol. 22:6498.
  4. Reverter D, et al. 2004. Structure. 12:1519.
  5. Butt TR, et al. 2005. Prot Expr Purif. 43:1.
  6. Yeh ETH, et al. 2006. J Biol Chem. 281:15869.
Gene ID
6612 View all products for this Gene ID
UniProt
View information about SUMO3 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 06.16.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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