- YJ5 (See other available formats)
- Regulatory Status
- Other Names
- G Protein-Coupled Receptor 177 (GPR177), WLS, Protein Wntless Homolog, Putative NF-Kappa-B-Activating Protein 373, C1orf139, Evi
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Wnt signaling plays important roles in embryonic development and cancer formation. Wntless (GPR177) is a putative G-protein coupled receptor that regulates Wnt's trafficking and secretion. It can regulate the expression, subcellular location, binding, and organelle-specific association of Wnt proteins. In mammals, Wntless is mainly expressed in submucosa, muscle, ganglia, and nerve cells of the large intestines. Overexpression of Wntless may activate the NF-κB signaling pathway in cancer cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Human Wntless protein
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- 0.5 mg/mL
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
WB - Quality tested
ICC, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The clone recognizes a ~65 kD protein of unidentified origin.
Additional reported applications (for relevant formats) include: immunoprecipitation (IP)1 and immunocytochemistry (ICC)1
(PubMed link indicates BioLegend citation)
- Coombs GS, et al. 2010. J. Cell Sci. 123:3357. (ICC, IP)
- Product Citations
AB_2562228 (BioLegend Cat. No. 655902)
- Three isoforms with 450 to 543 amino acids, predicted molecular weight of 45-65 kD; structure similar to the member of G-protein coupled receptor (GPCR) superfamily, with conserved YXXΦ endocytotic motif
- Regulates Wnt protein sorting and secretion; it plays a key role in the regulation of expression, subcellular localization, binding and organelle-specific association of Wnt proteins
- Interacts with WNT1, WNT3, WNT5A, and Vps35
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Signal Transduction
- Antigen References
1. Hardin J and King RS, et al. 2008. Curr. Opin. Genet. Dev. 18:362.
2. Wang LT, et al. 2012. Kaohsiung J. Med. Sci. 28:355.
3. Cornett B, et al. 2013. Dev. Biol. 2013. 379:38.
4. Das S, et al. 2012. Front. Biol. (Beijing). 7:587.
5. Stefater JA 3rd, et al. 2013. Blood 121:2574.
- Gene ID
- 79971 View all products for this Gene ID
- View information about Wntless on UniProt.org
|Purified anti-Wntless (GPR177)||YJ5||WB, ICC, IP|
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