Purified anti-TIMP-2 Antibody

Pricing & Availability
Clone
F27P3A4 (See other available formats)
Regulatory Status
RUO
Other Names
Metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2, TIMP2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
TIMP-2_WB
Western blot analysis of recombinant human TIMP-2 protein using anti-TIMP-2, clone F27P3A4.
  • TIMP-2_WB
    Western blot analysis of recombinant human TIMP-2 protein using anti-TIMP-2, clone F27P3A4.
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635401 25 µg 76,00€
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635402 100 µg 156,00€
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Description

TIMP-2 (Tissue inhibitor of metalloproteinase 2) is a nonglycosylated protein that belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 24 kD. The mechanisms of TIMPs inhibit angiogenesis appear to be specific for each member of the TIMP family. TIMP-2 binds to the surface of human endothelial cells through interaction with the integrin α3β1, and abrogates angiogenic factor induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. Clone F27P3A4 has been shown to be useful for western blotting, immunohistochemistry and ELISA of human, mouse, rabbit, chicken, zebrafish, and pig TIMP-2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rabbit, Chicken, Zebrafish, Swine (Pig, Porcine)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ovalbumin-conjugated synthetic Peptide DSGNDIYGNPI
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Murray G et al. 1998. Gut. 43:791.
  2. Fromique O, et al. 2008. J. Biol. Chem. 283:30549. PubMed
Product Citations
  1. Fromigué O, et al. 2008. J Biol Chem. 283:30549. PubMed
RRID
AB_2287490 (BioLegend Cat. No. 635401)
AB_2204826 (BioLegend Cat. No. 635402)

Antigen Details

Structure
Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 24 kD.
Distribution

Extracellular

Function
TIMP-2 binds to the surface of human endothelial cells through interaction with the integrin α3β1, and abrogates angiogenic factor induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition.
Interaction
MMP2, MMP8, MMP14, CD49C
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References

1. Stetler-Stevenson, et al. 1989. J. Biol. Chem. 264:17374

Gene ID
7077 View all products for this Gene ID
UniProt
View information about TIMP-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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