Purified anti-Parkin Antibody

Pricing & Availability
Clone
5C3/Parkin (See other available formats)
Other Names
PARK2, AR-JP, LPRS2, PDJ, PRKN, E3 ubiquitin-protein ligase parkin, Parkin 2, E3 ubiquitin ligase, parkinson disease protein 2, parkinson juvenile disease protein 2, Parkinson disease (autosomal recessive, juvenile) 2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5C3-Parkin_Purified_Parkin_Antibody_1_050919
Western blot of purified anti-Parkin antibody (clone 5C3/Parkin). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5C3-Parkin_Purified_Parkin_Antibody_1_050919
    Western blot of purified anti-Parkin antibody (clone 5C3/Parkin). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5C3-Parkin_Purified_Parkin_Antibody_2_050919
    Western blot of purified anti-Parkin antibody (clone 5C3/Parkin). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate. The blot was incubated with 2.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
865601 25 µg 72€
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865602 100 µg 188€
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Description

Parkin (also known as PARK2, AR-JP, PRKN) is a protein encoded by the PARK2 gene in humans. It is a component of the E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. Mutations in the PARK2 gene cause a familial form of Parkinson’s disease called autosomal recessive juvenile Parkinson’s disease (AR-JP).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Parkin is a 465 amino acid protein with an apparent molecular mass of ~ 52 kD.
Distribution

Tissue Distribution: Brain, heart, testis, and skeletal muscle.
Cellular Distribution: Cytosol, mitochondria, nucleus, and endoplasmic reticulum.

Function
Parkin in involved in protein ubiquitination, protein degradation, mitochondria function, and neurodegeneration.
Interaction
Ubiquitin, PINK1
Ligand/Receptor
BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Protein Misfolding and Aggregation, Ubiquitin/Protein Degradation
Molecular Family
Enzymes and Regulators, Ligases
Antigen References
  1. Tay SP, et al. 2018. J Biol Chem. 285: 29231-29238.
  2. Pawlyk AC, et al. 2003. J Biol Chem. 278(48):48120-8.
Gene ID
5071 View all products for this Gene ID
UniProt
View information about Parkin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.09.2019

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