- 10C2 (See other available formats)
- Regulatory Status
- Other Names
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.Product Details
- Antibody Type
- Host Species
- Fusion protein
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC - Verified
IHC-P - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry1.
(PubMed link indicates BioLegend citation)
- Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
- Product Citations
AB_2562473 (BioLegend Cat. No. 656801)
AB_2562474 (BioLegend Cat. No. 656802)
- 1621 amino acids with predicted molecular weight of 177 kD. The observed molecular weight is larger than 250 kD.
- Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
- Vimentin, alpha-internexin and FHOD3
- Cell Type
- Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Intermediate Filaments
- Antigen References
1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.
- Gene ID
- 10763 View all products for this Gene ID
- View information about Nestin on UniProt.org
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