Purified anti-mouse TCR Vγ4 Antibody

Pricing & Availability
Clone
49.2 (See other available formats)
Regulatory Status
RUO
Other Names
T Cell Receptor Vγ4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
49-2_PURE_TCR-Vy4_Antibody_082420
C57BL/6 splenocytes were stained with purified anti-mouse TCR Vγ4 (clone 49.2) (left) or purified mouse IgG1, κ isotype control (right) followed by rat anti-mouse IgG1 PE. Cells were co-stained with anti-CD3 FITC and anti-γδ TCR APC. Data shown are CD3+ gated cells.
  • 49-2_PURE_TCR-Vy4_Antibody_082420
    C57BL/6 splenocytes were stained with purified anti-mouse TCR Vγ4 (clone 49.2) (left) or purified mouse IgG1, κ isotype control (right) followed by rat anti-mouse IgG1 PE. Cells were co-stained with anti-CD3 FITC and anti-γδ TCR APC. Data shown are CD3+ gated cells.
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160602 500 µg 396,00€
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and β chain (TCR α/β) or a γ and δ chain (TCR γ/δ). TCR associates with CD3 to form a CD3/TCR complex. The CD3/TCR plays a key role in antigen recognition, signal transduction, and T cell activation. γδ T cells (gamma delta T cells) are a small subset of T cells that express a unique TCR. They are found in the thymus, peripheral lymphoid tissue, and peritoneum. Unlike αβ T cells, γδ T cells do not require antigen processing in order to recognize certain bacterial, lipid, and tumor antigens. The 49.2 antibody reacts specifically with mouse TCR Vγ4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/6 thymocytes expanded in vitro in anti-TCRγδ antibody coated plates
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Khairallah C, et al. 2015. PLoS Pathog. 11:e1004702.
  2. Perira P, et al. 2000. Eur J Immunol. 30:1988-97.
RRID
AB_2876572 (BioLegend Cat. No. 160602)

Antigen Details

Structure
Heterodimer with γ and δ chain
Distribution

Subset of γδ T cells

Function
Antigen recognition
Interaction
CD3
Cell Type
Lymphocytes, T cells, Thymocytes
Biology Area
Adaptive Immunity, Immunology, Innate Immunity
Molecular Family
TCRs
Gene ID
21638 View all products for this Gene ID
UniProt
View information about TCR Vgamma4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.10.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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