Purified anti-mouse/human IL-5 Antibody

Pricing & Availability
Clone
TRFK5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)
Isotype
Rat IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
504301 50 µg 56,00€
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504302 500 µg 92,00€
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Description

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Guinea Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially-purified T cell clone supernatant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
CyTOF® - Verified
IHC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-5 recombinant protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate when paired with TRFK4 (Cat. # 504402). To obtain a linear standard curve, serial dilutions of human IL-5 recombinant protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate when paired with JES1-5A10 (Cat. # 501002). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1,3,4 or ELISPOT2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.

Neutralization1,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).

Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections7, and immunocytochemistry.

Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  2. Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
  3. Swain, S., et al. 1990. J. Immunol. 145:3796.
  4. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  5. Assenmacher, M., et al. 1994. Eur. J. Immunol. 24:1097.
  6. Karulin, A., et al. 2000. J. Immunol. 164:1862.
  7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Fan, W. Y., et al. 2001. Exp. Biol. Med. 226:1045.
  9. Sitkauskiene, B., et al. 2005. Respir Res. 6:33.
Product Citations
  1. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  2. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  3. Trinh HKT, et al. 2020. Exp Mol Med. 52:253. PubMed
  4. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
RRID
AB_315325 (BioLegend Cat. No. 504301)
AB_315326 (BioLegend Cat. No. 504302)

Antigen Details

Structure
Cytokine; dimer; 40-50 kD (Mammalian)
Bioactivity
Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, eosinophils
Cell Targets
Eosinophils, B cells
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.

Gene ID
16191 View all products for this Gene ID 3567 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 08.01.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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