- W15110A (See other available formats)
- Other Names
- Interleukin-2-inducible T-cell kinase, T-cell-specific kinase, EMT, LYK, PSCTK2
- Mouse IgG1, κ
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- Product Citations
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IL2-inducible T-cell kinase (ITK) is an intracellular tyrosine kinase that is expressed in T cells. It regulates the proliferation, differentiation and development of conventional T cells and NK T cells. This protein has 620 amino acids and contains one PH domain for cell membrane recruitment, one protein kinase domain, and both SH2 and SH3 domains which are commonly found in intracellular kinases. Once T cells are activated, a series of phosphorylation recruits and activates ITK through autophosphorylation. The activated ITK can activate downstream intracellular calcium mobilization and NFAT nucleus translocation, and in turn, activate T cells. The phenotypes of mice deficient in ITK show severely affected immune response and development.Product Details
- Antibody Type
- Host Species
- Partial human ITK recombinant protein (1-200 a.a.) expressed in E. coli.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICFC, ICC - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is ≤ 5.0 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
AB_2616942 (BioLegend Cat. No. 687302)
- 620 amino acids with a predicted molecular weight of 71.8 kD. Contains a N-terminal PH domain, a SH2 domain, a SH3 domain, and a C-terminal catalytic kinase domain.
- ITK is a T-cell specific tyrosine kinase, regulating T cell proliferation and differentiation. It plays an important role in the adaptive immune response.
- Forms a homo-oligomer. Interacts with PPIA, FASLG, and VAV1.
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
1. Gomez-Rodriguez J, et al. 2016. Nat. Commun. 7:10857.
2. Cho HS, et al. 2015. J. Immunol. 195:4822.
3. Kannan A, et al. 2015. Eur. J. Immunol. 45:2276.
4. Ghosh S, et al. 2014. J. Clin. Immunol. 34:892.
5. Qi Q, et al. 2011. FEBS. J. 278:1970.
6. Prince AL, et al. 2009. Immunol. Rev. 228:115.
- Gene ID
- 3702 View all products for this Gene ID
- View information about ITK on UniProt.org
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