Purified anti-human CD170 (Siglec-5) Antibody

Pricing & Availability
1A5 (See other available formats)
Regulatory Status
VII 70443
Other Names
Mouse IgG1, κ
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Product Citations
Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 1A5_PE_051311
    Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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352002 100 µg 156€
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CD170, also known as Siglec-5, is a single pass transmembrane protein member of the immunoglobulin superfamily. On the cell surface, CD170 forms a 140 kD dimer. The cytoplasmic domain of Siglec-5 contains two ITIM motifs that recruit the tyrosine-phosphatases SHP-1 and SHP-2 after tyrosine-phosphorylation, which in turn results in the inhibition of cell signaling. Siglec-5 is expressed by granulocytes, monocytes/macrophages, subsets of lymphocytes, and a subset of activated dendritic cells. Siglec-5 binds α2,3- and α2,6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, Cross-Reactivity: Chimpanzee, Bonobo, Gorilla2
Antibody Type
Host Species
Extracellular region of Siglec-5
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 1A5 antibody cross-reacts with Siglec-144.  Additional reported applications (for the relevant formats) include: ELISA2.

Application References

(PubMed link indicates BioLegend citation)
  1. Cornish AL, et al. 1998. Blood 92:2123.
  2. Nguyen DH, et al. 2006. P. Natl. Acad. Sci. USA 103:7765. (FC, ELISA)
  3. Connolly NP, et al. 2002. Br. J. Haematol. 119:221.
  4. Angata T, et al. 2006. FASEB J. 20:1964.
  5. Avril T, et al. 2005. J. Biol. Chem. 280:19843.
AB_10896789 (BioLegend Cat. No. 352002)

Antigen Details

Member of the immunoglobulin superfamily, single pass transmembrane protein, forms a 140 kD dimer; cytoplasmic domain contains two ITIM motifs
Granulocytes, monocytes/macrophages, subsets of lymphocytes, and activated dendritic cells
Inhibitory receptor, cell adhesion
SHP-1, SHP-2
α2,3- and α2,6-linked sialic acid, glycophorin A
Cell Type
Granulocytes, Monocytes, Macrophages, Lymphocytes, Dendritic cells, B cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Soto PC, et al. 2010. J. Immunol. 184:4185.
2. Carlin AF, et al. 2009. J. Exp. Med. 206:1691.
3. Zhuravleva MA. 2008. J. Mol. Biol. 375:437.

Gene ID
8778 View all products for this Gene ID
View information about CD170 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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