Purified anti-Histone H3 Trimethyl (Lys9) Antibody (Previously Covance catalog# MMS-5079)

Pricing & Availability
6F12-H4 (See other available formats)
Regulatory Status
Other Names
Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
Covance Catalog# MMS-5079
Mouse IgG1
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Product Citations
Staining of Clone 6F12-H4 on frozen rat brain.
  • 6F12-H4_Purified_Histone_H3_Trimethyl_Lys9_Antibody_IHC_030315
    Staining of Clone 6F12-H4 on frozen rat brain.
Cat # Size Price Quantity Avail. Save
815601 100 µL 228,00€
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Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, Rat
Antibody Type
Host Species
This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, tri-methylated at Lysine-9.
Phosphate-buffered solution.
The antibody was purified by affinity chromatography.
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.


Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:100 - 1:1000*
IHC: 1:50 - 1:100

Tissue: Frozen sections
Pretreatment: None
Primary Incubation: 24 hours at 4°C

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

* Expected MW: 17 kD
WB Positive Control: HeLa cell lysate
IHC Positive Control: Rat Brain

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
AB_2564794 (BioLegend Cat. No. 815601)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Signal Transduction
Gene ID
8350 View all products for this Gene ID
View information about Histone H3 Trimethyl Lys9 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 04.14.2016

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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