Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody

Pricing & Availability
Clone
4B11B69 (See other available formats)
Other Names
P44/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
Isotype
Mouse IgG2b, κ
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Product Citations
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4B11B69_Purified_ERK1_2_Antibody_1_WB_070218
Total cell lysates (15 µg protein) from Jurkat cells without treatment (lane 1) and Jurkat cells starved overnight and then treated with PMA (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody (1:1000 dilution), clone 4B11B69 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody or a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody (lower). Lane M: Molecular weight ladder.
  • 4B11B69_Purified_ERK1_2_Antibody_1_WB_070218
    Total cell lysates (15 µg protein) from Jurkat cells without treatment (lane 1) and Jurkat cells starved overnight and then treated with PMA (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody (1:1000 dilution), clone 4B11B69 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody or a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody (lower). Lane M: Molecular weight ladder.
  • 4B11B69_Purified_ERK1_2_Antibody_2_IF_033115
    Untreated (left) and PMA-treated (20 nM PMA treatment for 10 minutes, right) mouse NIH-3T3 cells were stained with purified anti-ERK1/2 Phospho (Thr202/Tyr204) (clone 4B11B69) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green, Cat. No. 405310) antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
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675502 100 µg 200€
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Description

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration of 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565604 (BioLegend Cat. No. 675502)

Antigen Details

Structure
Human ERK1 (MAPK3) and ERK2 (MAPK1) encode 43, 40, 38kD and 41, 36kD protein kinases, respectively. Mouse ERK1 (MAPK3) and ERK2 (MAPK1) encode 43kD and 41kD protein kinases, respectively. ERK1 and ERK2 share high homology.
Distribution

Cytoplasm. Nucleus.

Function
ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.
Interaction
ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Phospho-Proteins, Postsynaptic proteins, Protein Kinases/Phosphatase
Antigen References

1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.

Gene ID
5594 View all products for this Gene ID
UniProt
View information about ERK1/2 Phospho on UniProt.org

Related FAQs

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