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Purified anti-Copper ATPase 1 Antibody

Pricing & Availability
Clone
L60/4 (See other available formats)
Regulatory Status
RUO
Other Names
ATPase Copper Transporting Alpha, Copper-Transporting ATPase 1, Menkes' protein, MNK, DSMAX, SAX3, MC1, MK
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
L60slash4_PURE_Copper-ATPase-1_Antibody_1_102021.png
IHC staining of purified anti-Copper ATPase 1 antibody (clone L60/4) on formalin-fixed paraffin-embedded human skin. Following antigen retrieval using 50 mM Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 10.0 µg/mL of either purified mouse IgG2b, κ isotype antibody (Cat. No. 402202) (panel A) or primary antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (red) (Cat. No. 405322) at 2.5 µg/mL. Nuclei were counterstained with DAPI (Cat. No. 422801), and the slides were mounted with ProLong™ Gold Antifade Mountant. Images were captured with a 40X objective. Scale: 50 µm.
  • L60slash4_PURE_Copper-ATPase-1_Antibody_1_102021.png
    IHC staining of purified anti-Copper ATPase 1 antibody (clone L60/4) on formalin-fixed paraffin-embedded human skin. Following antigen retrieval using 50 mM Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 10.0 µg/mL of either purified mouse IgG2b, κ isotype antibody (Cat. No. 402202) (panel A) or primary antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (red) (Cat. No. 405322) at 2.5 µg/mL. Nuclei were counterstained with DAPI (Cat. No. 422801), and the slides were mounted with ProLong™ Gold Antifade Mountant. Images were captured with a 40X objective. Scale: 50 µm.
  • L60slash4_PURE_Copper-ATPase-1_Antibody_2_102021.png
    IHC staining of purified anti-Copper ATPase 1 antibody (clone L60/4) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using 50 mM Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 10.0 µg/mL of purified mouse IgG2b, κ isotype antibody (Cat. No. 402202) (panel A) or 5.0 µg/mL primary antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (red) (Cat. No. 405322) at 2.5 µg/mL. Nuclei were counterstained with DAPI (Cat. No. 422801), and the slides were mounted with ProLong™ Gold Antifade Mountant. Images were captured with a 40X objective. Scale: 50 µm.
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601852 100 µg 132€
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Description

Copper ATPase 1, also known as ATP7A, is a copper-transporting ATPase which uses ATP hydrolysis to transport copper ions across membranes to regulate intracellular copper levels. As copper is an important trace nutrient required for cellular health, ATP7A is widely expressed in most tissues. For example, ATP7A expression in enterocytes in the small intestine controls dietary uptake of copper from digested food. Disruptions to the ATP7A gene result in Menkes disease, whose symptoms are caused by copper deficiency. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to amino acids 42-61 of recombinant human Copper ATPase 1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has been tested for WB and failed.

RRID
AB_2904445 (BioLegend Cat. No. 601852)

Antigen Details

Structure
ATP7A is a 1500 amino acid protein with a predicted molecular weight of 163 kD.
Distribution

Ubiquitously expressed in most cells except hepatocytes

Function
Regulate intracellular copper ion homeostasis via ATP hydrolysis
Antigen References
  1. Kaler, S 2011. Nat. Rev. Neurol. 7:25-29. 
Gene ID
538 View all products for this Gene ID
UniProt
View information about Copper ATPase 1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10.20.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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