Purified anti-β-actin Antibody

Pricing & Availability
Clone
Poly6221 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, cytoplasmic 1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
poly6221_PURE_betaactin_Antibody_1_WB_090616
Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG cells (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β-actin antibody (Poly6221). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection.
  • poly6221_PURE_betaactin_Antibody_1_WB_090616
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG cells (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β-actin antibody (Poly6221). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection.
  • poly6221_PURE_betaactin_Antibody_2_IF_062016
    Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.
  • poly6221_PURE_betaactin_Antibody_3_IHCP_102720
    Formalin-fixed paraffin-embedded human kidney tissue was stained with Poly6221 and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
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622101 50 µl (5 Western blots) 80,00€
 
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622102 200 µl (20 Western blots) 200,00€
 
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human β-actin.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat, Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide-KLH, NH2 terminus
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
ICC, IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry: Use a dilution range of 1:100~1:400. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  2. Joyce CW. 2006. J. Biol. Chem. 281:33053.
  3. Yanagiya T,et al.2007.Obesity.15:572.PubMed
  4. KishidaT,et al.2007.J. Immunol. 179:8554.PubMed
  5. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. PubMed
  6. Toltlt LJ,et al. 2008.J. Immunol. 181:2165. PubMed
  7. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. PubMed
  8. Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
  9. Rahman MK, et al. 2010. J. Immunol. 184:7247. PubMed
  10. Shikama Y, et al. 2011. Innate Immun. 17:3. PubMed
  11. Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:70. PubMed
  12. Piao X, et al. 2012. Sci Signal. 5:93. PubMed
  13. Ng CS, et al. 2013. J. Virol. 87:9511. PubMed
  14. Harvey BS, et al. 2014. Cytokine. 65:236. PubMed
  15. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
  16. Hua X, et al. 2015. PloS One. 10:128039. PubMed
Product Citations
  1. Grundmann M, et al. 2018. Nat Commun. 9:341. PubMed
  2. Zheng X, et al. 2018. Int J Mol Med. 128:1300. PubMed
  3. Wang D, et al. 2018. Immunity. 48:659. PubMed
  4. Merten N et al. 2018. Cell chemical biology. 25(6):775-786 . PubMed
  5. Panwar P, et al. 2018. Br J Pharmacol. 175:902. PubMed
  6. Kumarasinghe N, et al. 2019. BMC Res Notes. 12:33. PubMed
  7. Tsurusaki S, et al. 2019. Cell Death Dis. 10:449. PubMed
  8. Inao T, et al. 2019. Cancer Sci. 110:2690. PubMed
  9. Che L, et al. 2019. Exp Ther Med. 18:1745. PubMed
  10. Zhu S et al. 2017. Cancer cell. 32(3):310-323 . PubMed
  11. Naneix F et al. 2017. eNeuro. 4(3) pii: ENEURO. PubMed
  12. Al–Naggar IM, et al. 2019. J Gerontol A Biol Sci Med Sci. 74:325. PubMed
  13. Berdasco C, et al. 2020. Front Cell Infect Microbiol. 0.681944444. PubMed
  14. Tao HC, et al. 2020. Front Immunol. 11:148. PubMed
  15. Feddersen CR, et al. 2019. Cancer Res. 79:5074. PubMed
  16. Joyce C, et al. 2006. J Biol Chem. 281:33053. PubMed
  17. Kishida T, et al. 2007. J Immunol. 179:8554. PubMed
  18. Yanagiya T, et al. 2007. Obesity . 1.022222222. PubMed
  19. Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
  20. Sawada T, et al. 2008. J Biol Chem. 283:26820. PubMed
  21. Toltl L, et al. 2008. J Immunol. 181:2165. PubMed
  22. Ouimet M, et al. 2008. Arterioscler Thromb Vasc Biol. 28:1144. PubMed
  23. Rahman M, et al. 2010. J Immunol. 184:7247. PubMed
  24. Shikama Y, et al. 2011. Innate Immunity. 17:03. PubMed
  25. Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:F70. PubMed
  26. Piao X, et al. 2012. Sci Signal. 5:93. PubMed
  27. Ng C, et al. 2013. J Virol. 87:9511. PubMed
  28. Harvey B, et al. 2014. Cytokine. 65:236. PubMed
  29. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
  30. Hua X, et al. 2015. PLoS One. 10:128039. PubMed
  31. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  32. Wakatsuki S, et al. 2015. J Cell Biol. 211: 881 - 896. PubMed
  33. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  34. Checa M, et al. 2016. PLoS One. 11: 0150383. PubMed
  35. Takeda K, et al. 2017. Nat Commun. 8:14607. PubMed
  36. Agabiti S, Li J, Wiemer A 2017. Cell Death Dis. 10.1038/cddis.2017.101. PubMed
  37. Okimoto T, et al. 2020. Cancer Sci. 111:1910. PubMed
  38. Pfeil EM, et al. 2020. Molecular Cell. 80(6):940-954.e6. PubMed
  39. Taha A, et al. 2020. J Cell Physiol. 7094:235. PubMed
RRID
AB_315945 (BioLegend Cat. No. 622101)
AB_315946 (BioLegend Cat. No. 622102)

Antigen Details

Structure
Member of the actin family; 42 KD
Distribution

Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Interaction
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) that forms a two-stranded helix. Each actin can bind to four others.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Regulation
Three actin isoforms exist in vertebrates; alpha, found in muscle tissues; beta and gamma, found as cytoskeletal components and mediators of internal cell motility. Drugs such as cytochalasin disrupt the actin network.
Gene ID
11461 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 10.27.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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