Purified anti-AU1 Epitope Tag Antibody (Previously Covance catalog# PRB-130P)

Pricing & Availability
Clone
Poly29031 (See other available formats)
Regulatory Status
RUO
Other Names
AU1 epitope tag, DTYRYI epitope tag, DTYRYI tag
Previously
Covance Catalog# PRB-130P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
903101 100 µL 225,00€
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Description

AU1 is a commonly used epitope that is tagged onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Antibodies against AU1 epitope recognize proteins containing AU1 epitope tag fused to targeted proteins and allow for specific detection in several immunochemical assays such as western blotting, ELISAs and immunostaining techniques.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This polyclonal antibody was raised against the peptide DTYRYI (the same epitope as the monoclonal AU1 antibody).
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IHC, ICC, ELISA, Purification

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000-30,000
ICC: 1:200-2,000
ELISA: 1:100-400 (coating), 1:1,000-30,000 (primary)
 

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (ICC), immunocytochemistry and ELISA.

This antibody may be preferred over the monoclonal AU1 for experiments in murine systems or in experiments involving two proteins labeled with two different tags. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.


This product may contain other non-IgG subtypes.

Product Citations
  1. Kim GB et al. 2019. Cell. 179(1):251-267 . PubMed
  2. Bodur C, et al. 2018. EMBO J. 37:19. PubMed
RRID
AB_2565028 (BioLegend Cat. No. 903101)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about AU1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 01.25.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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