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PerCP/Cyanine5.5 anti-mouse/rat CD42d Antibody

Pricing & Availability
Clone
1C2 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet glycoprotein V, GPV, platelet gpV, CD42d antigen, CD42d
Isotype
Armenian Hamster IgG
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Product Citations
publications
1C2_PerCPCyanine55_CD42d_Antibody_101618
C57BL/6 platelets (resting) were stained with CD42d (clone 1C2) PerCP/Cyanine5.5 (filled histogram), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
  • 1C2_PerCPCyanine55_CD42d_Antibody_101618
    C57BL/6 platelets (resting) were stained with CD42d (clone 1C2) PerCP/Cyanine5.5 (filled histogram), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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148507 25 µg 109€
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148508 100 µg 278€
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Description

CD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX to form a receptor complex for von Willebrand factor on megakaryocytes and resting platelets. Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain of the GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex to release a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in Bernard-Soulier syndrome, a rare bleeding disorder.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1,3.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Takada K, et al. 1995. Hybridoma. 14:361. (IP)
  2. Saito M, et al. 1996. Stem Cells. 14:124. (FC)
  3. Sato N, et al. 2000. Hybridoma. 19:455. (IP)
RRID
AB_2564603 (BioLegend Cat. No. 148507)
AB_2564604 (BioLegend Cat. No. 148508)

Antigen Details

Structure
83 kD heterodimer composed of 2 polypeptide chains, an α chain (GP1BA) and a β chain (GP1BB).
Distribution

Resting platelets and megakaryocytes.

Function
CD42d is a component of the GPIb-V-IX complex, functions as a vWF receptor, and mediates vWF-dependent platelet adhesion to blood vessels.
Ligand/Receptor
CD42d.
Cell Type
Megakaryocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sivaraman B, et al. 2011. Biomaterials. 32:5365.
2. Berger G, et al. 1996. Blood. 87:1385.
3. Ravanat C, et al. 1997. Blood. 89:3253.
4. Andrews RK, et al. 1998. Biochemistry. 37:638.
5. Inoue O, et al. 2008. 283:16279.
6. Hickey MJ, et al. 1993. Proc. Natl. Acad. Sci. 90:8327.
7. Shimomura T, et al. 1990. Blood. 75:2349.
8. Wu G, et al. 1997. Blood. 90:2660.

Gene ID
14729 View all products for this Gene ID
UniProt
View information about CD42d on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 12.17.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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