PerCP/Cyanine5.5 anti-human CD74 (Cytoplasmic) Antibody

Pricing & Availability
Clone
Pin.1 (See other available formats)
Regulatory Status
RUO
Other Names
MHC Class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, MIF receptor, DHLAG, HLADG
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Pindot1_PerCPCy5_CD74_Antibody_1_111317
Human peripheral blood lymphocytes were surface stained with CD19 FITC, fixed and permeabilized, and then stained with CD74 (clone Pin.1) PerCP/Cyanine5.5 (top) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (bottom).
  • Pindot1_PerCPCy5_CD74_Antibody_1_111317
    Human peripheral blood lymphocytes were surface stained with CD19 FITC, fixed and permeabilized, and then stained with CD74 (clone Pin.1) PerCP/Cyanine5.5 (top) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (bottom).
  • Pindot1_PerCPCy5_CD74_Antibody_2_111317
See PerCP/Cyanine5.5 spectral data
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357607 25 tests 120,00€
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357608 100 tests 260,00€
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Description

CD74 is a type II transmembrane glycoprotein also known as MHC class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, and MIF receptor. CD74 exists in four isoforms with molecular masses of 33, 35, 41, and 43 kD, depending on genetic splicing. CD74 is primarily expressed on antigen presenting cells, including B cells, monocytes/macrophages, dendritic cells, and Langerhans cells. It is also expressed by activated T cells and activated endothelial and epithelial cells as well as carcinomas of lung, renal, gastric and thymic origin. The primary function of CD74 is intracellular sorting of MHC class II molecules and regulation of exogenous peptide loading onto MHC class II. It is also involved in the modulation of B cell differentiation and positive selection of CD4+ T cells. It has been reported that CD74 binds MIF (macrophage migration inhibitory factor) and signals through CD44 to regulate innate and adaptive immunity. It is also reported that H. pylori infection occurs through urease B binding of CD74 on gastric epithelial cells, inducing gastric epithelial cell apoptosis, NF-κB activation, and IL-8 production.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide DQKPVMDDQRDLISNNE, corresponding to amino acids 12-28 of the cytoplasmic tail of human CD74.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
RRID
AB_2721713 (BioLegend Cat. No. 357607)
AB_2721714 (BioLegend Cat. No. 357608)

Antigen Details

Structure
Type II transmembrane glycoprotein, 33, 35, 41, 43 kD
Distribution

B cells and other antigen presenting cells, activated T cells, activated endothelial and epithelial cells, and certain carcinomas

Function
Regulate loading of exogenous peptides onto MHC class II and B cell differentiation, involved in positive selection of CD4+ T cells.
Interaction
Associates with MHC II
Ligand/Receptor
HLA-DR, MIF, H. pylori urease B, associate with CD44
Cell Type
B cells, Antigen-presenting cells, T cells, Endothelial cells, Epithelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens
Antigen References

1. Koch N, et al. 1991. J. Immunol. 147:2643.
2. Wilson KM, et al. 1993. Immunology 79:331.
3. Moldenhauer G, et al. 1999. Immunology 96:473.
4. Shi X, et al. 2006. Immunity 25:595.
5. Beswick EJ, et al. 2006. Infect. Immun. 74:1148.
6. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication.

Gene ID
972 View all products for this Gene ID
UniProt
View information about CD74 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 0    Revision Date: 11.14.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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