PerCP anti-human CD45 Antibody

Pricing & Availability
2D1 (See other available formats)
Regulatory Status
Other Names
Leukocyte Common Antigen (LCA), T200
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) PerCP (filled histogram) or mouse IgG1, κ PerCP isotype control (open histogram).
  • 2D1_CD45_PerCP_FC_092915
    Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) PerCP (filled histogram) or mouse IgG1, κ PerCP isotype control (open histogram).
Compare all formats See PerCP spectral data
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368505 25 tests 93€
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368506 100 tests 225€
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CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human PBMC
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
  2. Csiba A, et al. 1984. Br. J. Cancer 50:699.
  3. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
  4. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations
  1. Oostindie SC, et al. 2022. Nat Biotechnol. :. PubMed
  2. Yang HL, et al. 2020. Am J Reprod Immunol. 84:e13261. PubMed
  3. Kreutmair S, et al. 2021. Immunity. . PubMed
  4. Wu H, et al. 2022. Biomolecules. 12:. PubMed
  5. Mulfaul K, et al. 2020. Cell Rep. 30:2209. PubMed
  6. Fu J, et al. 2020. Curr Protoc Pharmacol. 89:e76. PubMed
  7. Bäcker-Koduah P, et al. 2020. Ann Clin Transl Neurol. 1.422222222. PubMed
  8. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  9. Sawasdee N, et al. 2022. Int J Mol Med. 49:. PubMed
  10. Lozano-Rodríguez R, et al. 2022. Cell Rep. 38:110235. PubMed
  11. Li X, et al. 2021. Hemodial Int. 25:489. PubMed
  12. Zhang Q, et al. 2021. Sci Transl Med. 13:eabg6986. PubMed
  13. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  14. Weiss R, et al. 2021. Cells. 10:. PubMed
  15. Panigrahi S, et al. 2020. Eur J Immunol. 50:2055. PubMed
  16. Hu BS, et al. 2020. Cancer Cell Int. 1.189583333. PubMed
  17. Baliu-Piqu M, et al. 2021. eLife. 10:00. PubMed
  18. Yu Y, et al. 2017. Stem Cell Reports. 10.1016/j.stemcr.2017.07.016. PubMed
  19. Deng P, et al. 2020. Bone Res. 8:39. PubMed
AB_2566357 (BioLegend Cat. No. 368505)
AB_2566358 (BioLegend Cat. No. 368506)

Antigen Details

Type I transmembrane protein, 180 - 240 kD (multiple isoforms)

Hematopoietic cells, except circulating erythrocytes or platelets.

Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
Galectin-1, CD2, CD3, and CD4.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Protein Kinases/Phosphatase, TCRs
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
5788 View all products for this Gene ID
View information about CD45 on

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 10.23.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Spark Blue™ 550 anti-human CD45

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