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PE/Cyanine7 anti-human Granulysin Antibody

Pricing & Availability
Clone
DH2 (See other available formats)
Regulatory Status
RUO
Other Names
GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DH2_PECyanine7_Granulysin_Antibody_090120.png
Human peripheral blood lymphocytes surface stained with CD56 APC then intracellularly stained with Granulysin (clone DH2) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
  • DH2_PECyanine7_Granulysin_Antibody_090120.png
    Human peripheral blood lymphocytes surface stained with CD56 APC then intracellularly stained with Granulysin (clone DH2) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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348011 25 tests 128€
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348012 100 tests 296€
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Description

Granulysin is a member of the saposin-like protein family of lipid binding proteins (SAPLIP). It is expressed by NK cells and cytolytic T lymphocytes (CTL) as 9 kD and 15 kD forms. The 9 kD form is derived from the 15 kD form by cleavage at both amino- and carboxy-termini. Granulysin has broad cytolytic activities against a variety of tumor cells and microbes, including Gram-positive and -negative bacteria, fungi and parasites. It also functions as a chemoattractant for inflammatory cells and activates the cells expression of cytokines and chemokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant 9 kD granulysin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone DH2 recognizes both the 9 kD and 15 kD isoforms of Granulysin.1

ELISA Capture: To measure human granulysin, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated DH10 antibody (Cat. No. 526103) as the detection antibody. Recombinant Human Granulysin (ELISA Std.) (Cat. No. 552109) can be used as the protein standard.
Flow Cytometry: The fluorochrome-labeled DH2 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.
Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Hanson DA, et al. 1999. Mol. Immunol. 36:413.
  2. Spada FM, et al. 2000. J. Exp. Med. 191:937.
RRID
AB_2876660 (BioLegend Cat. No. 348011)
AB_2876660 (BioLegend Cat. No. 348012)

Antigen Details

Structure
Saposin-like protein family of lipid binding protein, 9 kD and 15 kD
Distribution

NK cells, cytolytic T cells

Function
Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Cell Type
NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Krensky AM. 2000. Biochem. Pharmacol. 59:317.
2. Deng A, et al. 2005. J. Immunol. 174:5243.
3. Kaspar AA, et al. 2001. J. Immunol. 167:350.
4. Krensky AM and Clayberger C. 2009. Tissue Antigens 73:193.

Gene ID
10578 View all products for this Gene ID
UniProt
View information about Granulysin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.01.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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