PE anti-human EGFR Antibody

Pricing & Availability
Clone
AY13 (See other available formats)
Regulatory Status
RUO
Other Names
Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1
Isotype
Mouse IgG1, κ
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Product Citations
publications
AY13_PE_090111
Human cervical cancer cell line HELA was stained with EGFR (clone AY13) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • AY13_PE_090111
    Human cervical cancer cell line HELA was stained with EGFR (clone AY13) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
352903 25 tests 94€
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352904 100 tests 259€
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Description

Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Non-small cell lung cancer (NSCLC) cell line NCI-H322
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Yamaguchi M, et al. 2009. The 15th Annual Meeting Japan Society of Gene Therapy. p1056. Abstract 92.
Product Citations
  1. Wang Y, et al. 2014. Biomaterials. 35:4297. PubMed
  2. Sreevalsan S, et al. 2020. EMBO Rep. 21:e50155. PubMed
  3. Kitamura Y, et al. 2021. Sci Adv. 7: . PubMed
  4. Halim L, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.079. PubMed
  5. Bak R and Proteus M. 2017. Cell Rep. 10.1016/j.celrep.2017.06.064. PubMed
  6. Wang JL, et al. 2022. Cancers (Basel). 14:. PubMed
  7. Lu T, et al. 2022. Nat Commun. 13:2576. PubMed
  8. Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed
  9. Aldeghaither DS, et al. 2019. Cancer Immunol Res. 7:230. PubMed
  10. Carnevale J, et al. 2022. Nature. 609:174. PubMed
  11. Grass G, et al. 2013. J Biol Chem. 288:26089. PubMed
  12. Ahn S, et al. 2019. Cancer Immunol Res. 0.828472222. PubMed
  13. Wing A, et al. 2018. Cancer Immunol Res. 6:605. PubMed
  14. Edinger N, et al. 2016. PLoS One. 11: 0162321. PubMed
  15. Han X, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.07.009. PubMed
  16. Brohl AS, et al. 2021. Cell Rep. 37:110047. PubMed
  17. Okada R, et al. 2021. EBioMedicine. 67:103345. PubMed
  18. Jarantow S, et al. 2015. J Biol Chem. 290: 24689 - 24704. PubMed
  19. Li G, et al. 2021. Mol Ther Oncolytics. 22:507. PubMed
  20. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
  21. Fischer A, et al. 2020. Toxins (Basel). 12:00. PubMed
RRID
AB_10898161 (BioLegend Cat. No. 352903)
AB_10896794 (BioLegend Cat. No. 352904)

Antigen Details

Structure
Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation
Distribution

Epithelial cells and endothelial cells; frequently found in epithelial carcinomas

Function
Controls cell growth and differentiation
Interaction
MAPK, Akt, JNK
Ligand/Receptor
Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
CD Molecules, Growth Factors
Antigen References

1. da Cunha Santos G, et al. 2011. Annu. Rev. Pathol. 6:49.
2. Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522.
3. Mano M and Humblet Y. 2008. Nat. Clin. Pract. Oncol. 5:415.
4. Pao W and Chmielecki J. 2010. Nat. Rev. Cancer 10:760.

Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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