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Pacific Blue™ anti-human IL-8 Antibody

Pricing & Availability
Clone
E8N1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-8, Neutrophil activating protein-1, Monocyte-derived neutrophil chemotactic factor (MDNCF), Neutrophil activating factor (NAF), Leukocyte adhesion inhibitor (LAI), Granulocyte chemotactic protein (GCP), CXCL8
Isotype
Mouse IgG1, κ
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Product Citations
publications
E8N1_PB_IL-8_Antibody_012820.png
LPS stimulated (6hrs) and monensin treated human peripheral blood monocytes surface stained with CD14 APC and intracellularly stained with IL-8 (clone E8N1) Pacific Blue™ (left) or mouse IgG1, κ Pacific Blue™ isotype control (right).
  • E8N1_PB_IL-8_Antibody_012820.png
    LPS stimulated (6hrs) and monensin treated human peripheral blood monocytes surface stained with CD14 APC and intracellularly stained with IL-8 (clone E8N1) Pacific Blue™ (left) or mouse IgG1, κ Pacific Blue™ isotype control (right).
Compare all formats See Pacific Blue™ spectral data
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511419 25 tests 123€
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511420 100 tests 278€
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Description

IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

ELISA Detection: The biotinylated E8N1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H8A5 (Cat. No. 511502) antibody as the capture antibody.
Note: For testing human IL-8 in serum or plasma, BioLegend's ELISA Max™ Sets are specially developed and recommended.

RRID
AB_2832821 (BioLegend Cat. No. 511419)
AB_2832822 (BioLegend Cat. No. 511420)

Antigen Details

Structure
CXC chemokine; 8.4 kD (Mammalian)
Ligand/Receptor
CXCR1 (CDw128); CXCR2
Bioactivity
Neutrophil/basophil chemoattractant; activates neutrophils, angiogenic factor
Cell Sources
Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
Cell Targets
Neutrophils, basophils, lymphocytes
Cell Type
Neutrophils, Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Baggiolini M, et al. 1994. Adv. Immunol. 55:97.
3. Schröder J, et al. 1992. Immunology Ser. 57:387.
4. Zwahlen R, et al. 1993. Intl. Rev. Expt. Pathol. 34:27.

Regulation
Upregulated by IL-1, IL-7, IL-17, IFN-γ, TNF-α, leukoregulin; downregulated by IL-4, TGF-ß, glucocorticoids, vitamin D3
Gene ID
3576 View all products for this Gene ID
UniProt
View information about IL-8 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01.28.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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