MojoSort™ Streptavidin Nanobeads

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RUO
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Product Citations
publications
StrepNano_1_051215
A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using the Streptavidin Nanobeads. Cells were stained with CD14 (clone M5E2) FITC.
  • StrepNano_1_051215
    A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using the Streptavidin Nanobeads. Cells were stained with CD14 (clone M5E2) FITC.
  • StrepNano_2_051215
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480015 100 µL 91€
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480016 1 mL 264€
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Description

Streptavidin Nanobeads can be used for positive or negative selection of targeted cells with biotin-conjugated antibodies. For positive selection, the magnetically labeled fraction is retained by the use of a magnetic separator. For negative selection, the untouched cells are collected by decanting the liquid in a clean tube. Either positive or negative selection cells can be used for downstream applications such as functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Streptavidin-conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

The concentration of Streptavidin Nanobeads to be used should be optimized by end users.

Application Notes

The Streptavidin Nanobeads are designed for the positive or negative isolation of cells when used with biotin-conjugated antibodies or similar reagents.

 

Additional Product Notes

View more applications data using this product to isolate mouse T and B cells and microglia in our Scientific Poster Library.


Watch a Scientific Poster video of this product.

Application References

(PubMed link indicates BioLegend citation)
  1. Guo H, et al. 2016. PLoS One. 11(3):e0150809. (PubMed)
Product Citations
  1. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  2. Downes DJ, et al. 2021. Nat Commun. 0.86875. PubMed
  3. Mitchell MI, et al. 2021. J Extracell Vesicles. 10:e12110. PubMed
  4. Rogers KJ, et al. 2021. J Leukoc Biol. 109:309. PubMed
  5. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  6. Song J, et al. 2021. Cancer Commun (Lond). 41:867. PubMed
  7. Knittel J, et al. 2022. STAR Protoc. 3:101632. PubMed
  8. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  9. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  10. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  11. Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed
  12. Kenney D, et al. 2022. Methods Mol Biol. 2580:211. PubMed
  13. Wang X, et al. 2022. Elife. 11:. PubMed
  14. Oudelaar AM, et al. 2020. Nat Commun. 2.348611111. PubMed
  15. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  16. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed

Antigen Details

Gene ID
NA

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 1    Revision Date: 05.14.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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