- Regulatory Status
- Other Names
- Multiplex assay, Flow cytometry, Inflammation, Flex set, Cytokines
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- Product Citations
Cytokines are small soluble glycoproteins that act as extracellular signaling molecuels and meidate communications between cells. They regulate immunity, inflammation and hematopoiesis as well as cell growth, migration, development and differentiation. Cytokines act in large tightly regulated networks where both the production and action of one cytokine is affected by the behavior of other cytokines. The accurate measurement of the expression profile of cytokines in a sample is critical to complete and in-depth understanding of disease progression and immune responses.
The LEGENDplex™ Mouse Inflammation Panel is a multiplex assay by using fluorescence–encoded beads suitable for the analysis on various flow cytometers. This panel allows simultaneous quantification of 13 mouse cytokines, including IL-1α, IL-1β, IL-6, IL-10, IL-12p70, IL-17A, IL-23, IL-27, MCP-1, IFN-β, IFN-γ, TNF-α, and GM-CSF. Most of cytokines in this panel are produced by innate immune cells, linking the innate and adaptive immunity and/or bystander cells. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples.
A LEGENDplex™ Mix and Match system is available to customize assays for specificities within this panel.
- Kit Contents
- Setup Beads 1: FITC Beads (1 ml)
- Setup Beads 2: PE Beads (1 ml)
- Setup Beads 3: Raw Beads (2 ml)
- Capture Beads
- Detection Antibody (3.5 ml)
- Standard Cocktail, Lyophilized (1 vial)
- SA-PE (3.5 ml)
- Matrix C, Lyophilized (1 vial)
- Assay Buffer (25 ml)
- Wash Buffer (25 ml)
- Filter Plate (1 plate)
- Plate Sealers (4 sheets)
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
- Additional Product Notes
View more applications data for this product in our Scientific Poster Library.
- Product Citations
- Materials Not Included
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Plate Shaker
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Gene ID
- 16160 View all products for this Gene ID 83430 View all products for this Gene ID 16175 View all products for this Gene ID 15978 View all products for this Gene ID 21926 View all products for this Gene ID 20296 View all products for this Gene ID 16159 View all products for this Gene ID 16160 View all products for this Gene ID 16176 View all products for this Gene ID 16153 View all products for this Gene ID 16193 View all products for this Gene ID 246779 View all products for this Gene ID 16171 View all products for this Gene ID 15977 View all products for this Gene ID 12981 View all products for this Gene ID
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.