LDH-Cytox™ Assay Kit

Pricing & Availability
Other Names
Lactate dehydrogenase assay
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Product Citations
publications
LDH_Cytox_Assay_Kit_Large_082117
Human FASL induced cytotoxicity in Jurkat cells is measured using both LDH-Cytox™ Assay Kit (OD 490nm) and Deep Blue Cell Viability™ Kit (RFU x 103).
  • LDH_Cytox_Assay_Kit_Large_082117
    Human FASL induced cytotoxicity in Jurkat cells is measured using both LDH-Cytox™ Assay Kit (OD 490nm) and Deep Blue Cell Viability™ Kit (RFU x 103).
Cat # Size Price Quantity Avail. Save
426401 500 tests 156€
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Description

LDH-Cytox Assay™ Kit is a kit for determination of cytotoxicity by measuring lactate dehydrogenase activity released from damaged cells. LDH catalyzes dehydrogenation of lactate to pyruvate thereby reducing NAD+ to NADH.  NADH reduces water-soluble tetrazolium salt in the presence of an electron mediator to produce an orange formazan dye.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Dye Mixture: Lyophilized, 1 bottle
  • Assay Buffer: 55 mL
  • Lysis Buffer: 5.5 mL
  • Stop Solution: 27.5 mL

Product Details

Formulation
This solution contains WST.
Storage & Handling
This kit is guaranteed for six months at 2-8°C.
Application

Cyt - Quality tested
FA, BA - Reported in the literature

Recommended Usage

For more information, please refer to the product manual.

Application References

(PubMed link indicates BioLegend citation)
  1. Jin SF, et al. 2015. Exp Cell Res. 339(2):289-99.
  2. Watanabe S, et al. 2016. Biochem Biophys Res Commun. 471:191.
  3. Wu L, et al. 2015. Am J Physiol Gastrointest Liver Physiol. 309:G695.
  4. Fukami T, et al. 2016. Biochem. Pharmacol. 116:153-61.
  5. Tanaka M, et al. 2016. J Pharmacol Sci. 131(3):162-71.
  6. Wakatsuki S, et al. 2015. J Cell Biol. 211(4):881-96.

Antigen Details

Biology Area
Cell Biology, Cell Proliferation and Viability, Mitochondrial Function, Signal Transduction
Gene ID
NA

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Go To Top Version: 2    Revision Date: 09.28.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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