FITC anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20_FITC_090507
SJL mouse splenocytes stained with A20 FITC
  • A20_FITC_090507
    SJL mouse splenocytes stained with A20 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
110705 50 µg 40,00€
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110706 500 µg 80,00€
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  2. Xu X, et al. 2018. Cell. 175:1336. PubMed
  3. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  4. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  5. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  6. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  7. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  8. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  9. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  10. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  11. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  12. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  13. Ise W, et al. 2018. Immunity. 48:702. PubMed
  14. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  15. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  16. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  17. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  18. Wan X, et al. 2018. Nature. 560:107. PubMed
  19. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  20. He L, et al. 2019. iScience. 0.790972222. PubMed
  21. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  22. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  23. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  24. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  25. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  26. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  27. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  28. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
  29. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  30. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  31. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
  32. Jameson K 2009. J Exp Med. 206:2253. PubMed
  33. Lee S, et al. 2009. J Immunol. 182:6753. PubMed
  34. Gay L, et al. 2013. Genes Dev. 27:98. PubMed
  35. Hata T, et al. 2013. J Immunol. 191:83. PubMed
  36. Lee P, et al. 2014. J Immunol. 192:178. PubMed
  37. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  38. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  39. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  40. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  41. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  42. Huang L, et al. 2015. PLoS Pathog. 11: 1005347. PubMed
  43. Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
  44. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  45. Fallet B, et al. 2016. Immunology. 1: eaah6817. PubMed
  46. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  47. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  48. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  49. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  50. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  51. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  52. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  53. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  54. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  55. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  56. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  57. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  58. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  59. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  60. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  61. Young K, et al. 2021. Cell Stem Cell. . PubMed
  62. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
RRID
AB_313494 (BioLegend Cat. No. 110705)
AB_313495 (BioLegend Cat. No. 110706)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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