- MEC14.7 (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Cells transfected with mouse CD34
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.
(PubMed link indicates BioLegend citation)
- Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
- Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
- Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
- Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
- Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
- Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
- Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
- Zaynagetdinov R., et al. 2011. J Immunol. 187:5703. PubMed.
- Product Citations
AB_10900980 (BioLegend Cat. No. 119321)
- Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow
- Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
- L-selectin, other selectins
- Cell Type
- Endothelial cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood 79:2288.
4. Baumhueter S, et al. 1994. Blood 84:2554.
- Gene ID
- 12490 View all products for this Gene ID
- View information about CD34 on UniProt.org
Related Pages & Pathways
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
Other FormatsView All CD34 Reagents Request Custom Conjugation
|Purified anti-mouse CD34||MEC14.7||FC,ICC,IHC-F,IP,WB|
|Biotin anti-mouse CD34||MEC14.7||FC,IHC-F|
|PE anti-mouse CD34||MEC14.7||FC|
|Alexa Fluor® 647 anti-mouse CD34||MEC14.7||FC,ICC|
|PE/Cyanine5 anti-mouse CD34||MEC14.7||FC|
|APC anti-mouse CD34||MEC14.7||FC|
|Brilliant Violet 421™ anti-mouse CD34||MEC14.7||FC,ICC|
|PE/Cyanine7 anti-mouse CD34||MEC14.7||FC|
|PerCP/Cyanine5.5 anti-mouse CD34||MEC14.7||FC|
|PE/Dazzle™ 594 anti-mouse CD34||MEC14.7||FC|
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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