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Biotinylated Recombinant Human SLAMF6-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
SLAMF6, SLAM family member 6, Activating NK receptor, NK-T-Bantigen, NTB-A
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Product Citations
publications
A. BIOTIN_RECOM_-Human_SLAMF6-Fc-Chimera_1_CF
When recombinant human SLAMF6-Fc (Cat No. 784606) is immobilized at 1 µg/mL, biotinylated recombinant human SLAMF6-Fc binds in a dose-dependent manner with ED50 range of 1 – 6 µg/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • A. BIOTIN_RECOM_-Human_SLAMF6-Fc-Chimera_1_CF
    When recombinant human SLAMF6-Fc (Cat No. 784606) is immobilized at 1 µg/mL, biotinylated recombinant human SLAMF6-Fc binds in a dose-dependent manner with ED50 range of 1 – 6 µg/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • B. BIOTIN_RECOM_-Human_SLAMF6-Fc-Chimera_2_CF
    Stability Testing for Biotinylated recombinant human SLAMF6. Biotinylated Recombinat human SLAMF6 was aliquoted in PBS at 0.2 mg/mL. One aliquotwas frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with recombinant human SLAMF6-Fc. The ED50 for this effect is 1 – 6 µg/mL.
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566704 25 µg 292€
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566706 100 µg 716€
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Description

SLAMF6, also known as NTB-A, is one of the members in the CD2/CD150 subfamily. SLAMF6 is a type I transmembrane glycoprotein and it is expressed on NK cells, T cells and B cells in humans. It is also found on macrophages, dendritic cells and granulocytes in mice. SLAMF6 is a self-ligand receptor. The homophilic interaction with SLAMF6-expressing cells and engagement of SLAMF6 by antibody-mediated cross-linking or recombinants trigger NK cell cytotoxicity, cytokine production and cell proliferation.  In addition, the homophilic interaction of SLAMF6 is involved in CD28 independent T cell co-stimulation, triggering Th1 cytokine production, and regulation of B cell tolerance. After stimulation, phosphorylated SLAMF6 associates with SAP/ SH2D1A and EAT-2/ SH2D1B adaptor proteins. Functional assays demonstrated that SAP is dispensable for SLAMF6-mediated cytotoxicity, but SLAMF6-mediated IFNγ production is impaired in the absence of SAP. In mice, SLAMF6, also called Ly108, plays an important role in CD4+ T cell responses and innate immunity to bacteria and parasites. In SLAMF6-deficient mice, CD4+ T cells and innate responses are defective. The SLAM family of receptors has been implicated in the pathophysiology of autoimmunity. SLAMF6 may censor self-reactive B cells as a potential regulator of tolerance checkpoints, safeguarding against autoimmunity. SLAMF6 is strongly linked to lupus susceptibility in mice and human patients. Therefore, SLAMF6 serves as a regulator of both innate and adaptive immune responses. Engagement of SLAMF6 along with CD3/TCR stimulation induces IL-17 production.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human SLAMF6 (NTB-A), amino acid (Leu28-Lys225) (Accession: # Q96DU3.3), with a linker (GS), a C-terminal human IgG (Pro100-Lys330) an Avi-tag, was expressed in CHO cells. Human SLAMF6-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 446 amino acid recombinant protein has a predicted molecular mass of approximately 50.23 kD. The DTT-reduced and non-reduced protein migrates at approximately 62 – 80 kD and 115 – 140 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Leu.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When recombinant human SLAMF6-Fc (Cat. No. 784606) is immobilized at 1 µ/mL, biotinylated recombinant human SLAMF6 binds in a dose-dependent manner with ED50 of 1 – 6 µg/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
CD2 subfamily
Distribution

Expressed in resting and activated NK cells, T cells, B cells; plasma membrane

Function
CD28 independent T cell co-stimulation, induction of NK cell cytotoxicity, regulation of B cell tolerance; plays an important role in CD4+ T cell responses, innate immunity to bacteria and parasites, and NK cell function
Interaction
SAP/ SH2D1A, EAT-2/ SH2D1B, Src kinase
Ligand/Receptor
SLAMF6 (self-ligand)
Bioactivity
Biotinylated recombinant human SLAMF6-Fc binds to immobilized recombinant human SLAMF6-Fc in a dose-dependent manner using a binding assay.
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, NK cells, T cells, Thymocytes
Biology Area
Adaptive Immunity, Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Bottino C, et al. 2001. J ExpMed. 194:235.
  2. Eissmann P, et al. 2006. J Immunol. 177:3170-7.
  3. Chatterjee M, et al. 2012. J Immunol. 188:1206-12.
  4. Howie D, et al. 2005. J Immunol. 174:5931-5.
  5. Valdez PA, et al. 2004. J Biol Chem. 279:18662-9.
  6. Kumar KR, et al. 2006. Science. 312:1665-9.
  7. Flaig RM, et al. 2004. J. Immunol. 172:6524-7.
  8. Wang N, et al. 2016. Clin Immunol. 173:19-26.
Gene ID
114836 View all products for this Gene ID
UniProt
View information about SLAMF6 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01.29.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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