Biotin anti-mouse LIF Antibody

Pricing & Availability
Poly5232 (See other available formats)
Regulatory Status
Other Names
Leukemia Inhibitory factor, HILDA, CDF, DIA, MLPLI
Goat Polyclonal IgG
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Product Citations
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523204 50 µg 300€
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Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a type I transmembrane ligand-binding subunit, the LIF-specific subunit gp190 (LIFR), and the type I transmembrane signal transducing subunit gp130, shared with the other members of the IL-6 family. It especially signals through STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissues. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. LIF can be upregulated by proinflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant Mouse LIF
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.1 - 1 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of LIF recombinant protein ranging from 1200 to 18.75 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

The biotinylated Poly5232 (Cat. No. 523204) antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the pure Poly5232 antibody (Cat. No. 523201) as the capture antibody and Recombinant Recombinant Mouse LIF (ELISA Std.) (Cat. No. 554009).

AB_2814446 (BioLegend Cat. No. 523204)

Antigen Details


Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells

Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemic cells
Embryonic stem cells
Gene ID
16878 View all products for this Gene ID
View information about LIF on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All LIF Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse LIF Poly5232 ELISA Capture
Biotin anti-mouse LIF Poly5232 ELISA Detection
LEAF™ Purified anti-mouse LIF Poly5232 Neut,WB
Go To Top Version: 1    Revision Date: 06.28.2019

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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