APC/Cyanine7 anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HIB19_APCCyanine7_CD19_Antibody_032019_updated.png
Human peripheral blood lymphocytes were stained with CD20 FITC and CD19 (clone HIB19) APC/Cyanine7 (left) or Mouse IgG1, ? isotype control (right)
  • HIB19_APCCyanine7_CD19_Antibody_032019_updated.png
    Human peripheral blood lymphocytes were stained with CD20 FITC and CD19 (clone HIB19) APC/Cyanine7 (left) or Mouse IgG1, ? isotype control (right)
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
302217 25 tests 104,00€
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302218 100 tests 212,00€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Chimpanzee, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed
  2. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  3. Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
  4. Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed
  5. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  6. Novakova L et al. 2018. Journal of neurochemistry. 146(3):322-332 . PubMed
  7. Shehata L, et al. 2019. Nat Commun. 10:1126. PubMed
  8. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  9. Tomellini E, et al. 2020. Cell Reports. 28(4):1063-1073.e5.. PubMed
  10. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  11. Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed
  12. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  13. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  14. Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed
  15. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  16. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  17. Joseph A, et al. 2010. J Virol. 84:6645. PubMed
  18. Simpfendorfer K, et al. 2012. Hum Mol Genet. 21:3918. PubMed
  19. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  20. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  21. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  22. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
  23. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  24. Deng C, et al. 2016. Diabetes Care. 39: 434 - 440. PubMed
  25. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  26. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  27. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  28. Tsuchida Y, et al. 2017. PLoS One. 10.1371/journal.pone.0169646. PubMed
  29. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  30. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  31. Yu B, et al. 2021. Cell. 184(7):1790-1803.e17. PubMed
  32. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  33. Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed
  34. Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed
  35. Kreutmair S, et al. 2021. Immunity. . PubMed
  36. Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
RRID
AB_314247 (BioLegend Cat. No. 302217)
AB_314248 (BioLegend Cat. No. 302218)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03.20.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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