APC anti-mouse IL-17A Antibody

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
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Product Citations
publications
TC11-18H10_APC_031808
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 APC
  • TC11-18H10_APC_031808
    PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 APC
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506915 25 µg 97€
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506916 100 µg 220€
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Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
  8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
  9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
  10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
  11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
  12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
  13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
  14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
  15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  2. Zongyi Y, et al. 2017. PLoS One. 10.1371/journal.pone.0189617. PubMed
  3. Chuang YM, et al. 2018. Front Cell Infect Microbiol. 8:31. PubMed
  4. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  5. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  6. Meyer Zu Horste G et al. 2018. Immunity. 48(3):556-569 . PubMed
  7. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  8. Pedraza–Zamora CP, et al. 2017. Parasite Immunol. 39:4. PubMed
  9. Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
  10. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  11. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  12. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  13. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  14. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  15. Liu J, et al. 2019. Immunity. 50:600. PubMed
  16. Lytle NK et al. 2019. Cell. 177(3):572-586 . PubMed
  17. Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
  18. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  19. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  20. Miranda PM, et al. 2018. Microbiome. 6:57. PubMed
  21. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  22. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  23. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  24. Jin C, et al. 2019. Cell. 176:998. PubMed
  25. Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed
  26. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  27. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  28. Kastelberg B, et al. 2020. PLoS Pathog. 16:e1008854. PubMed
  29. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  30. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  31. Li J, et al. 2010. Blood. 116:5615. PubMed
  32. Lebson L, et al. 2010. J Immunol. 185:7161. PubMed
  33. Robb R, et al. 2011. Blood. 118:3399. PubMed
  34. Bonnefoy F, et al. 2011. J Immunol. 186:5696. PubMed
  35. Wu H, et al. 2012. J Am Soc Nephrol. 23:1701. PubMed
  36. Mishra P, et al. 2013. Brain Behav Immun. 34:98. PubMed
  37. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  38. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  39. MaruYama T 2015. Biochem Biophys Res Commun. 464: 586-589. PubMed
  40. Shami A, et al. 2015. Sci Rep. 5: 13904. PubMed
  41. Zanvit P, et al. 2015. Nat Commun. 6: 8424. PubMed
  42. Arbelaez C, et al. 2015. J Immunol. 195: 1974-1983. PubMed
  43. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  44. Ma C, et al. 2016. Nature. 531:253-257. PubMed
  45. Nagashima H, et al. 2016. J Immunol. 196: 4082 - 4089. PubMed
  46. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  47. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  48. Zhang H, et al. 2017. Immunol Cell Biol. 10.1038/icb.2017.11. PubMed
  49. Stevanin M, et al. 2017. Eur J Immunol. 10.1002/eji.201646565. PubMed
  50. Webb LM, et al. 2020. J Clin Invest. 130:1683. PubMed
  51. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  52. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
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RRID
AB_536017 (BioLegend Cat. No. 506915)
AB_536018 (BioLegend Cat. No. 506916)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.

Other Formats

View All IL-17A Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture, CyTOF®, ELISPOT Capture, ICFC
FITC anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 488 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 647 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 700 anti-mouse IL-17A TC11-18H10.1 ICFC
APC anti-mouse IL-17A TC11-18H10.1 ICFC
Pacific Blue™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP/Cyanine5.5 anti-mouse IL-17A TC11-18H10.1 ICFC
PE/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 421™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 605™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 650™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 785™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 510™ anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A (Maxpar® Ready) TC11-18H10.1 ELISA Capture, CyTOF®
PE/Dazzle™ 594 anti-mouse IL-17A TC11-18H10.1 ICFC
APC/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 711™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP anti-mouse IL-17A TC11-18H10.1 ICFC
Ultra-LEAF™ Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture, CyTOF®, ELISPOT Capture, Neut, ICFC
Go To Top Version: 2    Revision Date: 10.16.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-mouse IL-17A

  • Purified anti-mouse IL-17A

  • FITC anti-mouse IL-17A

  • Alexa Fluor® 488 anti-mouse IL-17A

  • Alexa Fluor® 647 anti-mouse IL-17A

  • Alexa Fluor® 700 anti-mouse IL-17A

  • APC anti-mouse IL-17A

  • Pacific Blue™ anti-mouse IL-17A

  • PerCP/Cyanine5.5 anti-mouse IL-17A

  • PE/Cyanine7 anti-mouse IL-17A

  • Brilliant Violet 421™ anti-mouse IL-17A

  • Brilliant Violet 605™ anti-mouse IL-17A

  • Brilliant Violet 650™ anti-mouse IL-17A

  • Brilliant Violet 785™ anti-mouse IL-17A

  • Brilliant Violet 510™ anti-mouse IL-17A

  • Purified anti-mouse IL-17A (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse IL-17A

  • APC/Cyanine7 anti-mouse IL-17A

  • Brilliant Violet 711™ anti-mouse IL-17A

  • PerCP anti-mouse IL-17A

  • Ultra-LEAF™ Purified anti-mouse IL-17A

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