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- Clone
- W19011B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- G0/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
HeLa cells untreated (negative control, open histogram) or treated with 100 ng/mL PMA for 4 hours (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-FosB (clone W19011B) or Alexa Fluor® 647 rat IgG2a, κ isotype control (open histogram, dashed line) (representative histogram for untreated and treated cells) (Cat. No. 400526). -
HeLa cells untreated (panels A and B) or treated with 100 ng/mL of PMA (panels C and D) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour. Cells were then intracellularly stained with 5.0 µg/mL of Alexa Fluor® 647 anti-FosB (clone W19011B) (red) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Panels A and C represent Alexa Fluor® 647 channel; panels B and D represent merged Alexa Fluor® 647 and DAPI channels. Images were captured with a 60X objective. Scale bar: 50 µm
FosB is a member of the FOS family of proteins that dimerizes with proteins of the Jun family to form the AP-1 transcription factor complex. As part of AP-1, FosB plays a critical role in regulating cell differentiation, proliferation, and apoptosis. FosB is expressed in most tissues and has been particularly well-studied in the CNS. ΔFosB, a truncated splice variant of the FOSB gene, plays a critical role in the brain’s reward circuit plasticity, with significant impact on both natural and pathological behavior. FosB also functions as a tumor suppressor, and dysregulation of FosB activity or levels has been found in numerous cancers, including gastric, prostate and colorectal cancer.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full-length recombinant FosB protein
- Formulation
-
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) - Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC (test sizes) - Quality tested
ICC (µg size) - Quality tested - Recommended Usage
-
Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Methanol only and PFA + Methanol fix/perm methods were tested and produced inferior signal.
ICFC: Cells were fixed and permeabilizes using True Phos Buffer system
ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100 - RRID
-
AB_2941600 (BioLegend Cat. No. 600853)
AB_2941600 (BioLegend Cat. No. 600856)
AB_2941600 (BioLegend Cat. No. 600854)
Antigen Details
- Structure
- FosB is a 338 amino acid protein with a predicted molecular weight of 36kD.
- Distribution
-
FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue.
- Function
- FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation.
- Interaction
- Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G/C TCA) SWI/SNF chromatin remodeling complex, DPP4
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors
- Molecular Family
- Tumor Suppressors
- Antigen References
-
- Nestler E. 2015. Eur J Pharmacol. 753:66-72.
- Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61.
- Gene ID
- 2354 View all products for this Gene ID
- UniProt
- View information about FosB on UniProt.org
Related FAQs
Other Formats
View All FosB Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-FosB | W19011B | IHC-P,ICC,ICFC |
| Alexa Fluor® 647 anti-FosB | W19011B | ICFC,ICC |
| PE anti-FosB | W19011B | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-FosB
IHC staining of purified anti-FosB (clone W19011B) on formal... 
ICC staining of purified anti-FosB (clone W19011B) (panel A)... 
Hela cells treated with 100 ng PMA for 4 hours (positive tar... 
Untreated HeLa cells (negative target) were fixed with Fixat... -
Alexa Fluor® 647 anti-FosB
HeLa cells untreated (panels A and B) or treated with 100 ng... 
HeLa cells untreated (negative control, open histogram) or t... -
PE anti-FosB
HeLa cells untreated (negative control, open histogram) or t...
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