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Purified anti-Huntingtin Recombinant Antibody

Pricing & Availability
Clone
QA18A66 (See other available formats)
Regulatory Status
RUO
Other Names
HTT, HD, IT15, LOMARS
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A66_PURE_Huntingtin_RECOM_IHC-P_111121
IHC staining of anti-Huntingtin recombinant antibody (clone QA18A66) on formalin-fixed paraffin-embedded human brain (A, positive control) and skeletal muscle (B, negative control) tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissues were incubated with 5 µg/mL of recombinant anti-Huntingtin antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 μm
  • QA18A66_PURE_Huntingtin_RECOM_IHC-P_111121
    IHC staining of anti-Huntingtin recombinant antibody (clone QA18A66) on formalin-fixed paraffin-embedded human brain (A, positive control) and skeletal muscle (B, negative control) tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissues were incubated with 5 µg/mL of recombinant anti-Huntingtin antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 μm
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948901 25 µg $129
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948902 100 µg $323
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Description

Huntingtin (HTT) is ubiquitously expressed throughout the nervous tissue and is required for normal development. HTT is associated with vesicles and microtubules and may play a role in axonal transport. Huntington disease (HD) is caused by mutated HTT, which results from an abnormally expanded polyglutamine (polyQ) stretch derived from CAG repeats in its N-terminal. Aggregation of mutated HTT causes cellular toxicity and leads to cell death, therefore, reducing HTT aggregates may be a strategy to slow down disease progression. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Partial recombinant human Huntingtin protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Signals were detected on FFPE human brain tissues using this antibody.

RRID
AB_2904463 (BioLegend Cat. No. 948901)
AB_2904463 (BioLegend Cat. No. 948902)

Antigen Details

Structure
Huntingtin is a 3,144 amino acid protein with a molecular mass of 348 kD.
Distribution

Brain and peripheral nerves/Neuronal cells

Function
HTT protein maintains tissue homeostasis by regulating vesicular transport and recycling, cell division, ciliogenesis, endocytosis, autophagy, and gene transcription.
Interaction
HTT protein interacts with dynein/dynactin for vesicle transport and cell division, Hip1 for endocytosis, GTPase Rab11 for vesicle recycling, p62 for autophagy induction, and CBP, NeuroD, SP1, NF-κB, p53 for transcriptional regulations.
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroscience
Molecular Family
Microtubules, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Leavitt BR, et al. 2020. JAMA Neurol. 77:764-772.
  2. McColgan P, et al. 2019. Eur J Neurol. 25:24-34.
  3. Saudou F, et al. 2016. Neuron. 89:910-26.
Gene ID
3064 View all products for this Gene ID
UniProt
View information about Huntingtin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/11/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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