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Purified anti-Ankyrin G Antibody

Pricing & Availability
Clone
N106/20 (See other available formats)
Regulatory Status
RUO
Other Names
ANK3, Node Of Ranvier (Ankyrin G), MRT37
Isotype
Mouse IgG1, κ
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Product Citations
publications
N106slash20_PURE_AnkyrinG_Antibody_102021
Whole cell extracts (15 µg total protein) from human brain membrane tissue was heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified Ankyrin-G antibody (clone N106/20) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker.
  • N106slash20_PURE_AnkyrinG_Antibody_102021
    Whole cell extracts (15 µg total protein) from human brain membrane tissue was heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified Ankyrin-G antibody (clone N106/20) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker.
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601252 100 µg $165
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Description

Ankryin G is a submembranous scaffold protein expressed in a variety of tissues, including brain, heart, and muscle. In the neurons, Ankyrin G is localized in the axon initial segment and the nodes of Ranvier. Ankyrin G binds to βIV spectrin and actin to create a submembranous cytoskeletal network in axons. By itself, Ankyrin G controls the ion channel clustering and the maintenance of neuronal polarity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein of ~1000 amino acids of Ankyrin-G
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for IHC-P and failed.

RRID
AB_2904441 (BioLegend Cat. No. 601252)

Antigen Details

Structure
Scaffold protein
Distribution

Axon initial segments and nodes of Ranvier in neurons

Interaction
Ion channels
Antigen References
1. Huang C. and Rasband M., 2018. Ann N Y Acad Sci., 1420(1):46-61
2. Jenkins S. and Bennett V., 2001. J Cell Biol., 155(5):739-746
3. Leterrier C. et al., 2017. Front. Cell. Neurosci., 11:
Gene ID
288 View all products for this Gene ID
UniProt
View information about Ankyrin G on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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