APC/Fire™ 750 anti-human CD14

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone
M5E2
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
M5E2_APCFire750_ASR_CD14_Antibody_091521
Typical results from human peripheral blood monocytes stained either with M5E2 APC/Fire™ 750 used at 5 uL/test (filled histogram) or with an isotype control (open histogram).
  • M5E2_APCFire750_ASR_CD14_Antibody_091521
    Typical results from human peripheral blood monocytes stained either with M5E2 APC/Fire™ 750 used at 5 uL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
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982512 500 µL $376
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Formulation
Phosphate-buffered solution, pH7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
400 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

Suggested for Flow Cytometry

Disclaimer

WARNINGS AND PRECAUTIONS

  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
  6. Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Go To Top Version: 1    Revision Date: 09/22/2021

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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