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APC anti-human Folate Receptors α and β (FR-αβ) Antibody

Pricing & Availability
Clone
No.5/FOLR (See other available formats)
Regulatory Status
RUO
Other Names
FR-αβ
Isotype
Rat IgG2a, κ
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Product Citations
publications
No5slashFOLR_APC_Folate_Antibody_1_011218
Human epidermal carcinoma cells (KB cell line) were stained with APC anti-human FR-αβ (clone No.5/FOLR) (filled histogram) or APC Rat IgG2a, κ isotype control (open histogram).
  • No5slashFOLR_APC_Folate_Antibody_1_011218
    Human epidermal carcinoma cells (KB cell line) were stained with APC anti-human FR-αβ (clone No.5/FOLR) (filled histogram) or APC Rat IgG2a, κ isotype control (open histogram).
  • No5slashFOLR_APC_Folate_Antibody_2_011218
    Human FR-β transfected cells were stained with PE anti-human FR-αβ (clone No.5/FOLR) (filled histogram) or PE Rat IgG2a, κ isotype control (open histogram).
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391803 25 tests $142
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391804 100 tests $362
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Description

Human folate receptors alpha and beta (FR-α and FR-β) are members of a family of folate binding receptors that have diverse structural identities but mediate transport of folates into cells. The FR-α and FR-β isoforms are both GPI-anchored proteins with high affinity for folic acid. It is postulated that these receptors function as folate scavengers when folate supply is low or rapid cell growth requires elevated uptake of folate for methylation reactions including DNA biosynthesis. The expression of FR-α and FR-β are distinct in normal and malignant tissues. In normal tissue, FR-α is mainly expressed on the surface of a subset of polarized epithelial cells whereas its aberrant expression has been prominently correlated with malignancies of epithelial origin. Although the FR-β protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Monkey
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Folate beta transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

This clone reacts with both Human FOLR1 and FOLR2

Application References

(PubMed link indicates BioLegend citation)
  1. Nagai T, et al. 2015. Monoclon. Antib. Immunodiagn. Immunother. 34:181
RRID
AB_2721304 (BioLegend Cat. No. 391803)
AB_2721305 (BioLegend Cat. No. 391804)

Antigen Details

Structure
30 kD, seven disulfide bonds
Distribution

Epithelial cells and same type of tumor cells

Function
Transport of folate into cells
Ligand/Receptor
Folic Acid
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Antigen References

1. Elwood PC, 1989. J Biol Chem. 264:14893
2. Shen F, et al. 1994. Biochemistry. 33:1209-15
3. Yi YS, 2016. Immune Netw. 16:337

Gene ID
2348 View all products for this Gene ID 2350 View all products for this Gene ID
UniProt
View information about FR-alphabeta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/15/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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