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Purified anti-Siglec-E Antibody

Pricing & Availability
Clone
M1307D09 (See other available formats)
Regulatory Status
RUO
Other Names
CD170, Sialic acid-binding Ig-like lectin E, Siglec 9, Siglecl1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M1307D09_PURE_Siglec-E_Antibody_WB_122815
Western blot analysis of wild type mouse spleen (lane 1) and Siglec-E knock out mouse spleen (lane 2) using anti-Siglec E antibody (clone M1307D09) under non-reducing conditions. Anti-β actin antibody (clone poly6221) was used as a loading control. Siglec-E knock out mouse spleen tissue was kindly provided by Dr. Ajit Varki, UCSD.
  • M1307D09_PURE_Siglec-E_Antibody_WB_122815
    Western blot analysis of wild type mouse spleen (lane 1) and Siglec-E knock out mouse spleen (lane 2) using anti-Siglec E antibody (clone M1307D09) under non-reducing conditions. Anti-β actin antibody (clone poly6221) was used as a loading control. Siglec-E knock out mouse spleen tissue was kindly provided by Dr. Ajit Varki, UCSD.
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682802 100 µg $265
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Description

Siglecs (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region containing a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. The cytoplasmic tails of all siglecs have tyrosine based motifs with signaling functions. Siglecs are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Studies suggest that siglecs may participate in cell-cell interactions or act as receptors for the entry of viral or bacterial pathogens. Additionally, the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domain indicates that these molecules may play a role in the suppression of immunoreceptor signaling. Siglec-E is a mouse CD33-related siglec that selectively regulates early recruitment of neutrophils to the lungs in an acute lung inflammation induced by lipopolysaccharides. Siglec E-deficient mice exhibited exaggerated neutrophil recruitment that is reversible by using a blockade of the β2 integrin, CD11b. In addition, sialidase treatment of fibrinogen reverses the suppressive effects of Siglec-E on CD11b signaling. This suggests that sialic acid recognition by Siglec-E is required for its inhibitory function. These findings indicate that Siglec-E is an important negative regulator of neutrophil recruitment to the lung and β2 integrin-dependent signaling.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse Siglec-E produced in the 293E cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody works for Western blotting under non-reducing conditions.

Application References

(PubMed link indicates BioLegend citation)
  1. Siddiqui S, et. al. 2017. J. Biol. Chem. 292: 1029.
Product Citations
  1. Hsu YW, et al. 2021. J Biomed Sci. 28:5. PubMed
  2. Siddiqui S, et al. 2016. J Biol Chem. 292:1029-1037. PubMed
RRID
AB_2566696 (BioLegend Cat. No. 682802)

Antigen Details

Structure
569 amino acids with a predicted molecular weight of approximately 65 kD.
Distribution

Murine Siglec-E is expressed by neutrophils, natural killer (NK) cells, monocytes, macrophages, dendritic cells, and B1 cells.

Function
Siglec-E regulates neutrophil recruitment. Siglec-E signaling negatively regulates the LPS-induced production of TNF-α and IL-6 by macrophages. It is upregulated by a variety of TLR agonists.
Ligand/Receptor
α2,8-linked disialic acid.
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Siglec Molecules
Antigen References

1. McMillan SJ, et al. 2013. Blood 121:2084.
2. Bax M, et al. 2007. J. Immunol. 12:8216-24.
3. Angata T, Varki A. 2000. J. Biol. Chem. 275:22127.
4. Zhang JQ, et al. 2004. Eur. J. Immunol. 34:1175.
5. Yu Z, et al. 2001. Biochem. J. 353:483.

Gene ID
83382 View all products for this Gene ID
UniProt
View information about Siglec-E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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