BioLegend Product Reviews
Product: APC Annexin V
Catalog No.: 640920
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital

Staining Stimulated PBMC with Annexin V APC
Overall: Product Quality: Ease of Use:
Annexin V APC works well on stimulated PBMC.
Experimental Design

Application: Flow cytometry
Cells used: Stimulated human PBMC
Brief Protocol: PBMC were isolated from blood by centrifugation over Ficoll. PBMC were washed twice in PBS and stimulated for 3 days with anti-CD3/CD28. Cells were washed and stained for 15 minutes at room temperature with the recommended dilution of Annexin V APC in 100uL 1x binding buffer. Cells were washed twice with 2mL FACS buffer and analyzed on a BD LSR II.
Results Summary: Annexin V staining was excellent.
Additional Notes:
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Publication Status

This data has not been published.
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