Recombinant Human KGF (FGF-7) (Animal-Free)

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Other Names
Keratinocyte growth factor, Fibroblast growth factor 7 (FGF-7), HBGF-7
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711702 10 µg 172€
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Description

KGF is a heparin binding growth factor secreted by cells of mesenchymal origin. KGF acts in a paracrine manner by binding exclusively to a splicing variant of FGF receptor 2, FGFR2-IIIB. FGFR-IIIB is predominatly expressed by epithelial cells. This suggests that KGF may play an important role in mesenchymal-epithelial communication. KGF can stimulate proliferation, migration, and differentiation of epithelial cells in a variety of tissues. In skin, KGF can be strongly upregulated in dermal fibroblasts after skin injury. KGF can induce keratinocyte proliferation and migration. KGF can also protect epithelial cells from reactive oxygen species. In liver, KGF is required for adult liver regeneration. In brain, KGF is important for inhibitory synapse formation in the hippocampus. KGF-deficient mice are prone to epileptic seizures induced by chemical kindling in adults. KGF signaling is also important for hair follicle development. Injection of recombinant KGF into adult athymic nude mice can induce dose-dependent hair growth. In lung, KGF can protect animals from oxidant-induced injury. Taken together, KGF is a potential target for epithelial tissue repair.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human KGF, amino acids Cys32-Thr194 (Accession# Q6FGV5), was expressed in E. coli.
Molecular Mass
The 163 amino acid recombinant protein has a predicted molecular mass of approximately 18.9 kD. The predicted N-terminal amino acid is Cys.
Purity
>95%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Formulation
Lyophilized, carrier-free.
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in 1x PBS to a concentration of 0.5-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells is ≤ 10 ng/ml corresponding to a specific activity of ≥ 1 x 105 units/mg.
Application

Bioassay

Antigen Details

Structure
Growth factor
Distribution
KGF is widely expressed among cells of mesenchymal (stromal) origin.
Function
KGF is secreted by fibroblast and can stimulate proliferation of epithelial cells. KGF expression can be highly upregulated in various tissues after injury.
Interaction
Epithelial cells
Ligand/Receptor
FGF-7 receptor (FGFR2/IIIb).
Cell Type
Embryonic Stem Cells
Biology Area
Cell Proliferation and Viability, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Werner S, et al. 1998. Cytokine Growth Factor Rev. 9:153.
2. Werner S, et al. 1994. Science. 266:819.
23. Richardson GD, et al. 2009. Development. 136:2153.
24. Yen TT, et al. 2014. Protein Pept. Lett. 21:306.
25. Hsu YR, et al. 1999. Biochemistry. 38:2523.

Gene ID
2252 View all products for this Gene ID
UniProt
View information about KGF on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 05-06-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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